14TSB_SynBio A toolchest for rapid bootstrapping of novel chassis organisms

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New organisms for the expression of recombinant genes are needed. Organisms that have a greater resistance to theconditions in biocatalysis for the synthesis of pharmaceutical compounds are needed. We want to move from apetrochemical based chemical and pharmaceutical synthesis to a more bio-based economy and to do this we need muchbetter and more rapid tools for building biochemical and metabolic pathways in a new organism (chassis).The proposed project will develop rapid ways of getting high level expression in any new chassis organism. The varioussteps of enzyme expression will be dissected using our new tools and knowledge of how genes are expressed. This willlead to modular parts for the expression of biocatalysis genes and pathways in any new chassis. We will test thisdevelopment pathway in some selected chassis that are examples of novel organisms more fitted to the bioprocessconditions for the bio-synthesis of pharmaceutical compounds.