14TSB_SynBio A toolchest for rapid bootstrapping of novel chassis organisms

需要新的生物体来表达重组基因。需要对合成药物化合物的生物催化条件具有更大抵抗力的生物。我们希望从以石油化学为基础的化学和药物合成转向以生物为基础的经济，为此，我们需要更好、更快速的工具来在新生物体（底盘）中构建生化和代谢途径。提出的项目将开发在任何新的底盘生物体中获得高水平表达的快速方法。酶表达的各个步骤将使用我们的新工具和基因如何表达的知识进行剖析。这将导致模块化的部分表达的生物催化基因和途径在任何新的底盘。我们将在一些选定的底盘中测试这一开发途径，这些底盘是更适合药物化合物生物合成生物工艺条件的新生物体的例子。

New organisms for the expression of recombinant genes are needed. Organisms that have a greater resistance to theconditions in biocatalysis for the synthesis of pharmaceutical compounds are needed. We want to move from apetrochemical based chemical and pharmaceutical synthesis to a more bio-based economy and to do this we need muchbetter and more rapid tools for building biochemical and metabolic pathways in a new organism (chassis).The proposed project will develop rapid ways of getting high level expression in any new chassis organism. The varioussteps of enzyme expression will be dissected using our new tools and knowledge of how genes are expressed. This willlead to modular parts for the expression of biocatalysis genes and pathways in any new chassis. We will test thisdevelopment pathway in some selected chassis that are examples of novel organisms more fitted to the bioprocessconditions for the bio-synthesis of pharmaceutical compounds.