Bioprocessing of genetically engineered filamentous phages to underpin new therapeutic and industrial applications

基因工程丝状噬菌体的生物加工支持新的治疗和工业应用

• 批准号: BB/D521465/1
• 负责人: John Ward
• 金额: $ 30.94万
• 依托单位: University College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2006
• 资助国家: 英国
• 起止时间: 2006 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FD521465%2F1
• 关键词: Bioprocessing; genetically; engineered; filamentous; phages

The extensive knowledge of phage (also known as bacteriophages and bacterial viruses) genetics and physiology acquired in the past 50 years makes them an attractive option for development of products for pharmaceutical, diagnostic and nanotechnological applications. Modified phages are showing promise in the battle to defeat infectious and genetic diseases. For example, it has been shown that HIV epitopes displayed on a filamentous phage can elicit T-cell and B-cell responses offering a novel approach for vaccine design for a disease that affects approximately 6 million world-wide. Modified phages can be synthesised in high titres in E. coli and in principle processed by techniques readily applicable to considerable scale-up. Until a recently resurgence of interest in phages as anti-microbial agents there has been little research concern with large-scale processing since a few early studies 30 years ago. For this reason modern biochemical engineering and molecular biology concepts have yet to be brought together to address the issues involved. The proposed study will examine these. As the crisis in antibiotic drug resistance by microorganisms continues to develop it is also possible that phages will have an antibiotic role. Though this is not the primary focus of this research, the biochemical engineering studies proposed will bear on to this related field.

过去 50 年中获得的噬菌体（也称为噬菌体和细菌病毒）遗传学和生理学的广泛知识使其成为制药、诊断和纳米技术应用产品开发的有吸引力的选择。修饰噬菌体在战胜传染病和遗传性疾病的战斗中显示出了希望。例如，研究表明，丝状噬菌体上展示的 HIV 表位可以引发 T 细胞和 B 细胞反应，为针对影响全球约 600 万人的疾病的疫苗设计提供了一种新方法。修饰的噬菌体可以在大肠杆菌中以高滴度合成，并且原则上通过易于适用于相当大的放大的技术进行加工。直到最近人们对噬菌体作为抗微生物剂的兴趣重新兴起，自 30 年前的一些早期研究以来，很少有研究关注大规模加工。因此，现代生化工程和分子生物学概念尚未结合起来解决所涉及的问题。拟议的研究将检验这些。随着微生物对抗生素耐药性的危机不断发展，噬菌体也有可能发挥抗生素作用。虽然这不是本研究的主要焦点，但所提出的生化工程研究将涉及到这一相关领域。

项目成果

Determination of the survival of bacteriophage M13 from chemical and physical challenges to assist in its sustainable bioprocessing

• DOI: 10.1007/s12257-012-0776-9
• 发表时间: 2013-06-01
• 期刊: BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
• 影响因子: 3.2
• 作者: Branston, Steven D.;Stanley, Emma C.;Keshavarz-Moore, Eli
• 通讯作者: Keshavarz-Moore, Eli