Bioprocessing of genetically engineered filamentous phages to underpin new therapeutic and industrial applications

基因工程丝状噬菌体的生物加工支持新的治疗和工业应用

• 批准号: BB/D521465/1
• 负责人: John Ward
• 金额: $ 30.94万
• 依托单位: University College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2006
• 资助国家: 英国
• 起止时间: 2006 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FD521465%2F1
• 关键词: Bioprocessing; genetically; engineered; filamentous; phages

The extensive knowledge of phage (also known as bacteriophages and bacterial viruses) genetics and physiology acquired in the past 50 years makes them an attractive option for development of products for pharmaceutical, diagnostic and nanotechnological applications. Modified phages are showing promise in the battle to defeat infectious and genetic diseases. For example, it has been shown that HIV epitopes displayed on a filamentous phage can elicit T-cell and B-cell responses offering a novel approach for vaccine design for a disease that affects approximately 6 million world-wide. Modified phages can be synthesised in high titres in E. coli and in principle processed by techniques readily applicable to considerable scale-up. Until a recently resurgence of interest in phages as anti-microbial agents there has been little research concern with large-scale processing since a few early studies 30 years ago. For this reason modern biochemical engineering and molecular biology concepts have yet to be brought together to address the issues involved. The proposed study will examine these. As the crisis in antibiotic drug resistance by microorganisms continues to develop it is also possible that phages will have an antibiotic role. Though this is not the primary focus of this research, the biochemical engineering studies proposed will bear on to this related field.

在过去的50年里，对噬菌体(也称为噬菌体和细菌病毒)遗传学和生理学的广泛了解使它们成为开发制药、诊断和纳米技术应用产品的一个有吸引力的选择。经过修饰的噬菌体在战胜传染病和遗传病的战斗中显示出了希望。例如，已有研究表明，在丝状噬菌体上展示的HIV表位可以激发T细胞和B细胞反应，为一种影响全球约600万人的疾病提供了一种新的疫苗设计方法。经修饰的噬菌体可以在大肠杆菌中以高滴度合成，原则上可以通过易于应用于相当大规模的技术进行处理。直到最近，人们对噬菌体作为抗微生物制剂的兴趣重新抬头，自30年前的几项早期研究以来，对大规模加工的研究一直很少。出于这个原因，现代生化工程和分子生物学的概念尚未结合在一起，以解决所涉及的问题。拟议的研究将对这些问题进行研究。随着微生物对抗生素耐药性的危机继续发展，噬菌体也有可能发挥抗生素作用。虽然这不是本研究的主要重点，但所提出的生化工程研究将与这一相关领域有关。

项目成果

Determination of the survival of bacteriophage M13 from chemical and physical challenges to assist in its sustainable bioprocessing

• DOI: 10.1007/s12257-012-0776-9
• 发表时间: 2013-06-01
• 期刊: BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
• 影响因子: 3.2
• 作者: Branston, Steven D.;Stanley, Emma C.;Keshavarz-Moore, Eli
• 通讯作者: Keshavarz-Moore, Eli