EGF-R MODULATION AND RESPONSES IN COLON CARCINOMA
EGF-R MODULATION AND RESPONSES IN COLON CARCINOMA
批准号:
3194843
负责人:
LYNN C YEOMAN
金额:
$13.84万
依托单位国家:
美国
项目类别:
财政年份:
1990
资助国家:
美国
项目状态:
已结题
起止时间:
1990-07-01 至 1994-06-30
关键词:
DNA footprinting RNA antibody cell differentiation cell growth regulation chemical stability clone cells colon neoplasms complementary DNA electrophoresis epidermal growth factor gene induction /repression genetic library genetic promoter element genetic translation growth factor receptors high performance liquid chromatography immunofluorescence technique molecular cloning nuclear runoff assay nucleic acid hybridization nucleic acid probes phosphorylation plasmids protein tyrosine kinase protooncogene receptor expression transfection transforming growth factors
中文摘要
EGF-R在结肠癌中的调节和反应——研究提出
英文摘要
EGF-R Modulation and Responses in Colon Carcinoma - The research proposed
in this application will examine the role of epidermal growth factor
receptor (EGF-R) and transforming growth factor-alpha (TGF-a) levels of
expression upon the growth characteristics of individual human colon tumor
cell lines that have been grouped into three classes that are based upon
their biological characteristics. These three cell line classes draw upon
a bank of more than 20 human colon tumor cell lines that have been adapted
to growth in chemically defined, serum-free medium and have been
characterized in terms of their extent of differentiation, tumorigenicity,
growth in soft agarose and production of mucins. The relationship of EGF-R
expression and TGF-a production to cell growth will be studied by
determining the levels of receptor and factor expression in colon tumor
cell lines that diverge widely in their growth rate and extent of
differentiation, by varying the level of exogenous TGF-a and/or epidermal
growth factor (EGF), as well as by modulating the levels of functional EGF
receptor in at least two cell lines from each of the two most
phenotypically divergent of the 3 classes of human colon tumor cell lines.
This study will focus upon the following questions: (i) do the faster
growing colon tumor cell lines express more functional TGF-a, (ii) is the
level of cell-surface EGF-R directly related to level of EGF-R mRNA
expression, (iii) do TGF-a and EGF differ in their capacity to activate
EGF-R tyrosine specific protein kinase activity, (iv) do TGF-a and EGF
differ with regard to their capacity to produce different sites of amino
acid phosphorylation of the EGF-R, and (v) how does altered EGF-R number
and function influence colon cell growth and differentiation? By this
approach a better understanding of the relationship between natural levels
of TGF-a/EGF-R expression and colon tumor cell growth characteristics will
be achieved. Furthermore, the influence of EGF-R number on the basal cell
growth, cell growth requirements, and extent of differentiation for
individual colon cell line classes will be determined.
期刊论文(4)
专著(0)
科研奖励(0)
会议论文
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Transferrin and insulin enhance human colon tumor cell growth by differentiation class specific mechanisms.
转铁蛋白和胰岛素通过分化类特异性机制增强人结肠肿瘤细胞的生长。
DOI:
--
发表时间:
1996
期刊:
Oncology research.
影响因子:
--
作者:
[Yeoman,LC, Wan,CW, Zorbas,MA]
通讯作者:
Zorbas,MA
An autocrine model for cell- and matrix-associated fibroblast growth factor.
细胞和基质相关成纤维细胞生长因子的自分泌模型。
DOI:
--
发表时间:
1993
期刊:
Oncology research
影响因子:
3.1
作者:
[Yeoman,LC]
通讯作者:
Yeoman,LC
Growth control in a human colon carcinoma cell line mediated by cell-associated transforming growth factor-alpha (TGF alpha).
由细胞相关转化生长因子-α(TGF α)介导的人结肠癌细胞系的生长控制。
DOI:
10.1006/excr.1993.1119
发表时间:
1993
期刊:
Experimental cell research
影响因子:
3.7
作者:
[Zorbas,MA, Yeoman,LC]
通讯作者:
Yeoman,LC
Transforming growth factor-alpha production and autoinduction in a colorectal carcinoma cell line (DiFi) with an amplified epidermal growth factor receptor gene.
使用扩增的表皮生长因子受体基因转化结直肠癌细胞系 (DiFi) 中生长因子-α 的产生和自诱导。
DOI:
--
发表时间:
1993
期刊:
Cancer research
影响因子:
11.2
作者:
[Untawale,S, Zorbas,MA, Hodgson,CP, Coffey,RJ, Gallick,GE, North,SM, Wildrick,DM, Olive,M, Blick,M, Yeoman,LC]
通讯作者:
Yeoman,LC
CANCER PREVENTION IN MEDICAL EDUCATION PRACTICE
-
批准号:2761666
-
项目类别:
-
资助金额:$13.44万
-
财政年份:1999
-
负责人:LYNN C YEOMAN
-
依托单位:
CANCER PREVENTION IN MEDICAL EDUCATION PRACTICE
-
批准号:6376691
-
项目类别:
-
资助金额:$14.6万
-
财政年份:1999
-
负责人:LYNN C YEOMAN
-
依托单位:
CANCER PREVENTION IN MEDICAL EDUCATION PRACTICE
-
批准号:6173526
-
项目类别:
-
资助金额:$14.01万
-
财政年份:1999
-
负责人:LYNN C YEOMAN
-
依托单位:
EGF-R MODULATION AND RESPONSES IN COLON CARCINOMA
-
批准号:3194838
-
项目类别:
-
资助金额:$10.89万
-
财政年份:1990
-
负责人:LYNN C YEOMAN
-
依托单位:
EGF-R MODULATION AND RESPONSES IN COLON CARCINOMA
-
批准号:3194841
-
项目类别:
-
资助金额:$1.24万
-
财政年份:1990
-
负责人:LYNN C YEOMAN
-
依托单位:
EGF-R MODULATION AND RESPONSES IN COLON CARCINOMA
-
批准号:3194842
-
项目类别:
-
资助金额:$13.17万
-
财政年份:1990
-
负责人:LYNN C YEOMAN
-
依托单位:
NON-HISTONE CHROMATIN PROTEINS OF TUMORS AND OTHER CELLS
-
批准号:3961389
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:LYNN C YEOMAN
-
依托单位:
NON-HISTONE CHROMATIN PROTEINS OF TUMORS AND OTHER CELLS
-
批准号:4690233
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:LYNN C YEOMAN
-
依托单位:
NON-HISTONE CHROMATIN PROTEINS OF TUMORS AND OTHER CELLS
-
批准号:3937506
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:LYNN C YEOMAN
-
依托单位:
国内基金
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