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中文摘要
翻译
结肠癌中EGF-R的调节和反应--研究建议 在本应用中将考察表皮生长因子的作用 血管内皮细胞生长因子受体(EGF-R)和转化生长因子-α(TGF-α)水平 人结肠癌个体生长特性的表达 已经被分成三类的细胞系,它们基于 它们的生物学特征。这三个单元线类别利用 已适应的20多个人结肠肿瘤细胞系的库 在化学定义的无血清培养基中生长,并已 以其分化程度、致瘤性、 在软琼脂中生长并产生粘蛋白。表皮生长因子受体的相互关系 表达和转化生长因子-α的产生对细胞生长的影响将通过 结肠癌组织中受体和因子表达水平的测定 在生长速度和程度上差异很大的细胞系 通过改变外源转化生长因子-α和/或表皮的水平进行分化 生长因子(EGF)以及调节功能性EGF水平 受体在至少两个细胞系中分别来自两个最多的 3种人结肠肿瘤细胞系的表型分化。 这项研究将集中在以下问题上:(I)做得更快 生长中的结肠肿瘤细胞株表达更多功能性的转化生长因子-α,(Ii)是 细胞表面EGF-R水平与EGF-R mRNA水平直接相关 表达,(Iii)转化生长因子-α和表皮生长因子的激活能力是否不同 EGF-R酪氨酸特异性蛋白激酶活性,(Iv)DO转化生长因子-α和EGF 它们产生不同位置氨基的能力不同 EGF-R的酸性磷酸化,以及(V)EGF-R数量是如何改变的 和功能是否影响结肠细胞的生长和分化?借此 更好地理解自然层次之间的关系 转化生长因子-α/表皮生长因子受体的表达与结肠癌细胞生长特性的关系 才能实现。此外,EGF-R数量对基底细胞的影响 生长、细胞生长要求和分化程度 将确定单个结肠细胞系的类别。
英文摘要
EGF-R Modulation and Responses in Colon Carcinoma - The research proposed in this application will examine the role of epidermal growth factor receptor (EGF-R) and transforming growth factor-alpha (TGF-a) levels of expression upon the growth characteristics of individual human colon tumor cell lines that have been grouped into three classes that are based upon their biological characteristics. These three cell line classes draw upon a bank of more than 20 human colon tumor cell lines that have been adapted to growth in chemically defined, serum-free medium and have been characterized in terms of their extent of differentiation, tumorigenicity, growth in soft agarose and production of mucins. The relationship of EGF-R expression and TGF-a production to cell growth will be studied by determining the levels of receptor and factor expression in colon tumor cell lines that diverge widely in their growth rate and extent of differentiation, by varying the level of exogenous TGF-a and/or epidermal growth factor (EGF), as well as by modulating the levels of functional EGF receptor in at least two cell lines from each of the two most phenotypically divergent of the 3 classes of human colon tumor cell lines. This study will focus upon the following questions: (i) do the faster growing colon tumor cell lines express more functional TGF-a, (ii) is the level of cell-surface EGF-R directly related to level of EGF-R mRNA expression, (iii) do TGF-a and EGF differ in their capacity to activate EGF-R tyrosine specific protein kinase activity, (iv) do TGF-a and EGF differ with regard to their capacity to produce different sites of amino acid phosphorylation of the EGF-R, and (v) how does altered EGF-R number and function influence colon cell growth and differentiation? By this approach a better understanding of the relationship between natural levels of TGF-a/EGF-R expression and colon tumor cell growth characteristics will be achieved. Furthermore, the influence of EGF-R number on the basal cell growth, cell growth requirements, and extent of differentiation for individual colon cell line classes will be determined.
期刊论文(4)
专著(0)
科研奖励(0)
会议论文
Transferrin and insulin enhance human colon tumor cell growth by differentiation class specific mechanisms.
转铁蛋白和胰岛素通过分化类特异性机制增强人结肠肿瘤细胞的生长。
DOI: --
发表时间: 1996
期刊: Oncology research.
影响因子: --
作者: [Yeoman,LC, Wan,CW, Zorbas,MA]
通讯作者: Zorbas,MA
An autocrine model for cell- and matrix-associated fibroblast growth factor.
细胞和基质相关成纤维细胞生长因子的自分泌模型。
DOI: --
发表时间: 1993
期刊: Oncology research
影响因子: 3.1
作者: [Yeoman,LC]
通讯作者: Yeoman,LC
Growth control in a human colon carcinoma cell line mediated by cell-associated transforming growth factor-alpha (TGF alpha).
由细胞相关转化生长因子-α(TGF α)介导的人结肠癌细胞系的生长控制。
DOI: 10.1006/excr.1993.1119
发表时间: 1993
期刊: Experimental cell research
影响因子: 3.7
作者: [Zorbas,MA, Yeoman,LC]
通讯作者: Yeoman,LC
Transforming growth factor-alpha production and autoinduction in a colorectal carcinoma cell line (DiFi) with an amplified epidermal growth factor receptor gene.
使用扩增的表皮生长因子受体基因转化结直肠癌细胞系 (DiFi) 中生长因子-α 的产生和自诱导。
DOI: --
发表时间: 1993
期刊: Cancer research
影响因子: 11.2
作者: [Untawale,S, Zorbas,MA, Hodgson,CP, Coffey,RJ, Gallick,GE, North,SM, Wildrick,DM, Olive,M, Blick,M, Yeoman,LC]
通讯作者: Yeoman,LC
CANCER PREVENTION IN MEDICAL EDUCATION PRACTICE
  • 批准号:
    2761666
  • 项目类别:
  • 资助金额:
    $13.44万
  • 财政年份:
    1999
  • 负责人:
    LYNN C YEOMAN
  • 依托单位:
CANCER PREVENTION IN MEDICAL EDUCATION PRACTICE
  • 批准号:
    6376691
  • 项目类别:
  • 资助金额:
    $14.6万
  • 财政年份:
    1999
  • 负责人:
    LYNN C YEOMAN
  • 依托单位:
CANCER PREVENTION IN MEDICAL EDUCATION PRACTICE
  • 批准号:
    6173526
  • 项目类别:
  • 资助金额:
    $14.01万
  • 财政年份:
    1999
  • 负责人:
    LYNN C YEOMAN
  • 依托单位:
EGF-R MODULATION AND RESPONSES IN COLON CARCINOMA
  • 批准号:
    3194838
  • 项目类别:
  • 资助金额:
    $10.89万
  • 财政年份:
    1990
  • 负责人:
    LYNN C YEOMAN
  • 依托单位:
国内基金
海外基金
免标记CRISPR-RNA适配体与门逻辑分子诊断新方法研究
  • 批准号:
    2026JJ50010
  • 项目类别:
    省市级项目
  • 资助金额:
    --
  • 批准年份:
    2026
  • 负责人:
    应站明
  • 依托单位:
RNA m6A修饰通过调控FDX1介导的铜死亡参与补阳还五汤抗脑缺血再灌注损伤作用机制的研究
  • 批准号:
    2026JJ81091
  • 项目类别:
    省市级项目
  • 资助金额:
    --
  • 批准年份:
    2026
  • 负责人:
    刘亮
  • 依托单位:
基于合成生物标志物的超多重RNA数字化检测平台用于肿瘤精准诊断和分期评估
  • 批准号:
  • 项目类别:
    省市级项目
  • 资助金额:
    --
  • 批准年份:
    2026
  • 负责人:
    程子译
  • 依托单位:
Dead-box解旋酶DDX23通过调控RNA高级结构促进肝癌细胞恶性生物学行为的分子机制研究
  • 批准号:
    JCZRLH202600588
  • 项目类别:
    省市级项目
  • 资助金额:
    --
  • 批准年份:
    2026
  • 负责人:
  • 依托单位: