IMMORTALIZATION OF PRIMARY EPITHELIAL CELLS BY AD5 12S

AD5 12S 对原代上皮细胞的永生化

• 批准号: 3195079
• 负责人: MARGARET P QUINLAN
• 金额: $ 6.31万
• 依托单位: UNIVERSITY OF TENNESSEE HEALTH SCI CTR
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-06-01 至 1995-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3195079
• 关键词: RNA splicing; cell line; cellular oncology; epithelium; gene expression; molecular oncology; neoplasm /cancer genetics; neoplastic transformation

The long term goals are to identify the changes in the program of cellular gene expression induced by the 12S gene that results in primary-epithelial cell immortalization and the mechanism(s) by which they occur. Knowing how a cell becomes immortalized is important for our ultimate understanding of the multistep conversion of a normal cell to the tumorigenic transformed phenotype. Although most human neoplasms are of epithelial origin, very little is known about their transformation. Since the 12S gene specifically affects this cell type, it provides a means to study then progression at the cellular and molecular level. Much is known about the first exon of 12S, therefore the investigator will concentrate on the second exon, whose expression is also required for immortalization. The investigator will perform a detailed genetic analysis of the second exon to determine which region(s) and function(s) are important for immortalization. Cellular genes whose expression is modulated by the presence of this region, will be identified.

长期目标是确定细胞程序的变化， 由12S基因诱导的基因表达，导致原代上皮细胞 细胞永生化及其发生的机制。知道如何 细胞的永生化对于我们最终理解 正常细胞向致瘤性转化细胞的多步转化 表型虽然大多数人类肿瘤是上皮起源的， 人们对它们的转变知之甚少。因为12S基因 影响这种细胞类型，它提供了一种方法来研究然后进展， 细胞和分子水平。 关于12S的第一个外显子已知很多，因此研究人员将 集中在第二个外显子上，其表达也是 永生研究人员将进行详细的基因分析 第二个外显子，以确定哪些区域和功能（S）， 对永生很重要表达受调控的细胞基因 这个区域的存在，将被识别。