GENETIC ANALYSIS OF HEMOPOIETIC HISTOCOMPATIBILITY

造血组织相容性的遗传分析

• 批准号: 3197273
• 负责人: ICHIRO NAKAMURA
• 金额: $ 18.17万
• 依托单位: STATE UNIVERSITY OF NEW YORK AT BUFFALO
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-04-01 至 1994-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3197273
• 关键词: chromosome walking; genetic library; genetic mapping; genetic recombination; genetically modified animals; haploidy; hematopoietic tissue transplantation; histocompatibility; histocompatibility gene; homologous transplantation; immunogenetics; laboratory mouse; major histocompatibility complex; molecular cloning; natural killer cells; northern blottings; nucleic acid probes; restriction fragment length polymorphism; southern blotting; transfection

DESCRIPTION: (Adapted from the applicant's abstract) The long-term objective of this project is to understand the genetic mechanism(s) underlying the natural resistance to hemopoietic allografts. As an initial approach towards the stated objective, three specific aims are proposed: 1) to determine the location of the Hh-1 locus in the H-2-b and H-2-d chromosomes by determining restriction fragment length polymorphism (RFLP) in a panel of H-2S/D recombinant haplotypes, 2) to identify and isolate the Hh-1 genes, using human and murine probes for the H-2S/D interval and H-2-b and H-2-d cosmid libraries, and 3) to initiate the characterization of the genes thus identified. The proposed approach should provide new insight into the genetic mechanism underlying natural resistance and the recognition function of NK cells. In addition, these studies should help identify homologous genes in man, improve the prospect of clinical bone marrow transplantation, and possibly develop NK cell-mediated cancer immunotherapy.

描述：（改编自申请人的摘要）长期 该项目的目标是了解遗传机制 是对造血同种异体移植物的天然抵抗力的基础。 作为初始 为了实现既定目标，提出了三个具体目标： 1)确定Hh-1位点在H-2-b和H-2-d中的位置 通过确定限制性片段长度多态性 (RFLP) 来确定染色体 在一组 H-2S/D 重组单倍型中，2) 鉴定和分离 Hh-1 基因，使用人和鼠探针进行 H-2S/D 间隔和 H-2-b 和 H-2-d 粘粒文库，以及 3) 开始表征 由此鉴定出的基因。 所提出的方法应该提供新的见解 深入了解自然抵抗力的遗传机制和 NK细胞的识别功能。 此外，这些研究应该有助于 鉴定人体同源基因，改善临床骨科前景 骨髓移植，并可能发展为 NK 细胞介导的癌症 免疫疗法。