GENETIC ANALYSIS OF HEMOPOIETIC HISTOCOMPATIBILITY

描述：(改编自申请人的摘要)长期 本项目的目标是了解遗传机制(S) 对同种异体造血移植的天然抵抗力。作为首字母 为了实现所述目标，提出了三个具体目标： 1)确定HH-1基因在H-2-b和H-2-d中的位置 用限制性片段长度多态(RFLP)测定染色体 在一组H-2S/D重组单倍型中，2)鉴定和分离 利用人和小鼠的H-2S/D间期和H-2-b探针检测HH-1基因 和H-2-d粘粒文库，以及3)开始表征 这样识别出的基因。建议的方法应该提供新的见解 自然抗性的遗传机制和 NK细胞的识别功能。此外，这些研究应该会有所帮助。 识别人类同源基因，提高临床骨应用前景 骨髓移植，并可能发展为NK细胞介导的癌症 免疫疗法。

DESCRIPTION: (Adapted from the applicant's abstract) The long-term objective of this project is to understand the genetic mechanism(s) underlying the natural resistance to hemopoietic allografts. As an initial approach towards the stated objective, three specific aims are proposed: 1) to determine the location of the Hh-1 locus in the H-2-b and H-2-d chromosomes by determining restriction fragment length polymorphism (RFLP) in a panel of H-2S/D recombinant haplotypes, 2) to identify and isolate the Hh-1 genes, using human and murine probes for the H-2S/D interval and H-2-b and H-2-d cosmid libraries, and 3) to initiate the characterization of the genes thus identified. The proposed approach should provide new insight into the genetic mechanism underlying natural resistance and the recognition function of NK cells. In addition, these studies should help identify homologous genes in man, improve the prospect of clinical bone marrow transplantation, and possibly develop NK cell-mediated cancer immunotherapy.