The diagnostic window for detection of viruses infecting salmon in erythrocytes.

鱼感染病毒的诊断通常是通过检测鱼组织中病毒的核酸来进行的。这种方法忽视了越来越多的证据表明，这些病毒可以在血液中复制。如果血液可以作为诊断样本，它将简化并可能加快诊断测试。我们想要探索感染鲑鱼的五种病毒(ISAV，SAV，PRV，PMCV，IPMV)在鲑鱼红细胞中的复制。我们想要评估这些病毒中哪些在红细胞中表现出良好的复制能力，以及是否可以得出结论，在活鲑鱼中检测它们的诊断窗口。我们还想探索病毒感染红细胞是否会引起免疫反应，这将表明首次接触感染病原体的红细胞也参与了对感染的一线防御。我们想要监测的另一个特征是受感染的红细胞中诱导的形态变化。如果这些病毒在红细胞中的复制得到证实，将有助于简化病毒诊断。此外，为了使检测标准化，我们希望为鱼类诊断实验室的分子诊断的外部质量评估开发材料。这是绝对必要的，因为认证服务无法评估个别实验室的表现，也无法将个别实验室的表现与其他实验室进行比较。简而言之，在人类感染病毒的诊断中已经确立的质量评估的整个概念，在鱼类病毒的实验室现场测试中缺失。为此，我们将来自格拉斯哥的QMCD纳入我们的财团，该财团在开发EQA面板方面具有长期的专业知识。总体来说，该项目的结果将有助于简化和优化感染鲑鱼的病毒的分子检测。

Diagnosis of viruses infecting fish is often done by detecting the nucleic acids of the viruses in fish tissues. This approach ignores increasing evidence that these viruses can replicate in blood. If blood could be used as a diagnostic sample it would simplify and possibly also speed up diagnostic testing.We want to explore the replication of five viruses infecting salmon (ISAV, SAV, PRV,PMCV, IPMV) in salmon erythrocytes. We want to assess which of these viruses show good replication in erythrocytes and if conclusions for a diagnostic window for their detection in live salmon can be drawn. We also want to explore if virus infection of the erythrocytes illicits an immune response, which would show that erythrocytes which are in first contact with the infectious agents are also involved in front line defense against infection. Another feature we would like to monitor are the morphological changes induced in infected erythrocytes.If replication of these viruses in erythrocytes can be confirmed it would lead the way to simplifying virus diagnostics. To additionally standardize testing we want to develop material for external quality assessments of molecular diagnostics in fish diagnostic laboratories. This is absolutely necessary since accreditation services can not assess how individual laboratories are performing nor are individual laboratories able to compare their performance to others. In short the whole concept of quality assessment, which is well established in the diagnostics of viruses infecting humans, is missing in the laboratory scene testing for fish viruses. To this end we have included QMCD from Glasgow into our consortium which has a long standing expertise in developing EQA panels.Overall the outcomes of this project will help to simplify and to optimize molecular detection for viruses infecting salmon.