Developing the Mupirocin QS system of P fluorescens into an efficient and economical way to control industrial production of high value products

将荧光磷莫匹罗星 QS 系统开发为控制高价值产品工业生产的高效且经济的方法

• 批准号: BB/M028739/1
• 负责人: Christopher Thomas
• 金额: $ 107.16万
• 依托单位: University of Birmingham
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FM028739%2F1
• 关键词: Developing; Mupirocin; QS; system; fluorescens

Industrial production of high value products by bacteria is a vital part of the modern biotechnology and pharmaceutical industries. Competition to produce such products as cheaply as possible means that the costs of the specialist chemicals added to fermenters to switch on production need to be controlled. The mupIRX gene system in Pseudomonas fluorescens regulates genes encoding the Mupirocin biosynthetic pathway, allows switch on of gene expression by an internal signal that accumulates as the bacteria grow and has already been manipulated to yield benefits for antibiotic production. We will define the elements of this control system to allow us to construct systems to put the production of other high value products under the control of this system. The innovation is in exploiting the native control system in an established and proven industrial host strain.

利用细菌生产高价值产品是现代生物技术和制药工业的重要组成部分。以尽可能低的成本生产此类产品的竞争意味着需要控制添加到发酵罐中以启动生产的专用化学品的成本。荧光假单胞菌中的mupIRX基因系统调节编码Mupirocin生物合成途径的基因，允许通过细菌生长过程中积累的内部信号开启基因表达，并且已经被操纵以产生抗生素生产的益处。我们将定义这个控制系统的要素，使我们能够构建系统，将其他高价值产品的生产置于该系统的控制之下。创新之处在于利用已建立和证明的工业宿主菌株的本地控制系统。

项目成果

High quality genome annotation and expression visualisation of a mupirocin-producing bacterium.

• DOI: 10.1371/journal.pone.0268072
• 发表时间: 2022
• 期刊: PloS one
• 影响因子: 3.7