Glycoenzymes for Bioindustries

自然界中有丰富的糖酶，但只有少数酶活性用于工业过程，主要是用于大批量生产的碳水化合物降解酶，如用于烘焙/洗涤剂/生物燃料的淀粉酶/木聚糖酶/纤维素酶/氧化酶。在生物制剂等高价值制造应用中存在的例子甚至更少：一个例子是治疗性Cerezyme的生产，其中α -新氨基酶用于脱糖基化，暴露末端Man残基，提高其靶向性。一个主要的障碍是适当的酶的可用性，这可能限制在过程开发的早期阶段的利用，从而减少涉及糖酶的成功过程的数量。本项目旨在通过汇集一些创新的高通量方法来发现生物催化剂，从而克服当前糖酶可用性的限制。我们已经收集了20种不同的糖酶活性，包括各种糖基转移酶、多糖修饰酶、糖苷酶和糖氧化酶，这将构成研究的基础。通过反复的生物催化剂发现、表征和开发，我们的目标是将不同类型的糖酶推向市场，这些酶酶可以很容易地用于最终用户的筛选。

There are a wealth of glycoenzymes in nature but only a small number of enzymatic activities are used in industrial processes, primarily carbohydrate degrading enzymes used in high volume manufacturing such as amylases/xylanases/cellulases/oxidases used in baking/detergents/biofuels. Even fewer examples exist in high value manufacturing applications such as biologics: one example is the production of the therapeutic Cerezyme where alpha-neauramindase is used to deglycosylate exposing terminal Man residues improving its targeting. A major hurdle is the availability of appropriate enzymes which can limit utilisation in the early stage of process development thus reducing the number of successful processes involving glycoenzymes. The present project aims to overcome the current limitations of glycoenzyme availability by bringing together a number of innovative high-throughput approaches to biocatalyst discovery. We have assembled a list of 20 different glycoenzyme activities including various glycosyltransferases, polysaccharide-modifying enzymes, glycosidases and sugar oxidases that will form the basis of the research. Through iterative rounds of biocatalyst discovery, characterisation and development we aim to bring to market panels of different glycoenzyme classes that are readily available for screening by end users.