MOLECULAR TAXONOMY OF NON-HEMOLYTIC STREPTOCOCCI

非溶血性链球菌的分子分类学

• 批准号: 3220679
• 负责人: ALAN L COYKENDALL
• 金额: $ 8.36万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-08-01 至 1988-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3220679
• 关键词: Streptococcus; Streptococcus infection; Streptococcus mitis; Streptococcus salivarius; Streptococcus sanguis; bacterial DNA; bacterial endocarditis; bacterial genetics; genetic strain; microorganism classification; microorganism culture; radiotracer; systematic biology

The non-hemolytic or "viridans" streptococci cause endocarditis, abscesses of the brain and internal organs, and septicemias. The presence of S. bovis in feces correlates with colon malignancies. Yet there is no universally accepted taxonomy of these bacteria. This complicates efforts to identify them. In many laboratories they remain unidentified. A better taxonomy, based on genetic relationships, would foster accurate and timely identification, facilitate epidemiology, and promote the development of new technology based on DNA "probes" for the rapid identification of these streptococci. Therefore, strains identified as, resembling, or possibly belonging to S. mitior, S mitis, S. milleri, S. sanguis, S. salivarius, and S. bovis will be collected from large clinically oriented microbiological laboratories. Recognized stock strains and type strains as well as recent clinical isolates will be acquired. The bacteria will be characterized by standard cultural and biochemical tests. DNA will be isolated from these cocci after cell crops are lysed with a combination of penicillin, lysozyme, and detergent. The base compositions of the DNAs will be determined by thermal denaturation, and the DNA base sequence similarities will be assessed by DNA-DNA hybridization on membrane filters or in solution. The streptococcal strains will be grouped into species based on the similarities of their DNA and then defined phenotypically by the biochemical and cultural characteristics which correlate best with the gentically defined species. In this way, the taxonomy of this important group of bacteria can be established on a molecular foundation and the relationships of these species can be defined more strictly. The overall goal is to aid clinicians in their efforts to help patients afflicted with these streptococci.

非溶血性或“绿色”链球菌可引起心内膜炎、脓肿 大脑和内脏，以及败血症。S.的存在。 粪便中的牛磺酸与结肠癌有关。然而，没有 被普遍接受的这些细菌的分类。这使努力复杂化。 来确认他们的身份。在许多实验室中，它们仍然没有被识别出来。更好的 基于遗传关系的分类学将有助于准确和及时地 识别，促进流行病学，促进新的 基于DNA“探针”的快速鉴定技术 链球菌。因此，被鉴定为、类似于或可能是 属于米氏链球菌S、米勒氏链球菌、血链球菌、唾液链霉菌和 牛链球菌将从大型临床导向微生物中收集 实验室。公认的库存菌株和模式菌株以及最近的 将获得临床分离株。这种细菌的特征将是 标准的培养和生化测试。DNA将从这些细胞中分离出来 在细胞作物用青霉素混合物裂解后的球菌， 溶菌酶和洗涤剂。DNA的碱基组成将是 由热变性和DNA碱基序列相似性决定 将通过膜过滤器上的DNA-DNA杂交或在 解决方案。链球菌菌株将根据以下条件进行分类 它们的DNA相似之处，然后通过 生物化学和文化特征与 基因定义的物种。通过这种方式，这一重要的 细菌群可以建立在分子基础上，而 这些物种的亲缘关系可以被更严格地定义。整体而言 目标是帮助临床医生努力帮助患有 这些链球菌。