STUDIES ON ENAMEL PROTEINS

釉质蛋白是一种复杂的蛋白质混合物，它可能扮演着 在釉质形成过程中的中心作用，以及 蛋白质的数量、它们的结构和出现的顺序以及 牙釉质形成过程中的消失对于理解 这一过程。根据目前可用的技术，这些蛋白质 从牙釉质基质中增溶可分为15至25个组分。 目前尚不清楚这些组件中的每一个是否代表单个 蛋白质，单个基因的产物，或者成分是否 是相互关联的。可能，多个组件派生自 作为生理的一部分，有限数量的蛋白质的分解 在提取过程中或在用于分离蛋白质的提取过程中。 这项提案的总体目标是描述牙釉质的特征 蛋白质和它们的基因，并试图定义它们在 成釉作用。针对牛牙釉质蛋白的单抗将是 制备并用于确定免疫学和结构关系 在蛋白质之间。这些抗体也将被用来确定 单个蛋白质出现和消失的时间 并通过免疫组织化学和免疫组织化学方法对这些蛋白进行原位定位 超微结构技术。

Enamel proteins are a complex mixture of proteins which probably play a central role in the process of amelogenesis, and the determination of the number of proteins, their structure and sequence of appearance and disappearance during enamel formation is essential to an understanding of that process. With presently available techniques, the proteins solubilized from enamel matrix can be separated into 15 to 25 components. It is not known whether each of these components represents a single protein, the product of an individual gene, or whether the components are interrelated. Possibly, the multiple components are derived from the breakdown of a limited number of proteins either as part of a physiologic process or during the extraction procedures used to isolate the proteins. The overall objective of this proposal is to characterize the enamel proteins and their genes and to attempt to define their role in amelogenesis. Monoclonal antibodies to bovine enamel proteins will be prepared and used to define the immunologic and structural relationships between the proteins. These antibodies will also be used to determine the time of appearance and disappearance of individual proteins during amelogenesis and to localize the proteins in situ by immuno histologic and ultrastructural techniques.