TOXIC EFFECT OF CHLORINATED AND PYRETHROID PESTICIDES

在这项研究项目中，我们建议研究氯化的毒性效应。 拟除虫菊酯类农药主要作用于钙调节过程。 神经系统、肝脏和心脏。主要的实验动物 被使用的是老鼠，但老鼠、豚鼠、仓鼠和一些 无脊椎动物物种也将被使用。主要研究的化合物有 滴滴涕、毒杀芬、氯丹、狄氏艾氏剂、乙草胺、环氧乙草胺 丙烯菊酯、氯氰菊酯、氰戊菊酯、氯氰菊酯和溴氰菊酯。在 就肝脏而言，主要的重点将放在长期 这些化学物质对肝细胞钙转运功能的影响。 此外，还将努力研究它们对缝隙连接的影响 已知由钙浓度控制，因为它的 功能。这些农药对细胞的影响程度 认可和沟通将被确定。在这种情况下 神经系统，我们计划研究它们对各种钙的影响 钠钙交换等调节系统，特别是部分 与三磷酸腺苷利用和钙镁三磷酸腺苷酶有关。在后者的情况下 其与钙调蛋白相互作用及递质的变化 释放，并对神经分泌进行检查。至于环二型 我们计划检查氯化前驱剂对GABA-氯的影响 渠道系统。重点将放在以下研究上 印防己毒素受体。该项目还将涵盖以下领域 农药对心脏生化的影响，特别是与其相关的 对钙调节的影响。

In this research project we propose to study toxic effects of chlorinated and pyrethroid pesticides mainly on calcium regulating processes in the nervous system, the liver and the heart. The main experimental animals to be used is the rat, but the mouse, the guinea pig, the hamster and some inverterbrate species will also be used. The compounds mainly studied are DDT, toxaphene, chlordane, dieldrin aldrin, neptachlor, neptachlor epoxide, allethrin, permethrin, fenvalerate cypermetnrin and decamethrin. In the case of the liver, the major emphasis will be placed on the long term effects of these chemicals on calcium transport functions of hepatocytes. Also an effort will be made to study their effects on the gap-junction which is known to be controlled by calcium concentration for its functions. The extents of the influence of these pesticides on cell-cell recognition and communication will be determined. In the case of the nervous system, we plan to study their influence on various calcium regulating systems such as Na-Ca exchange, particularly the portion relating to ATP utilization, and on Ca-Mg ATPase. In the latter case changes in its interaction with calmodulin as well as in transmitter release, and neurosecretion will be examined. As for cyclodience-type chlorinated presticides we plan to examine their effects on GABA-chloride channel systems. A particular emphasis will be placed on the studies on the picrotoxinin receptor. This project will also cover the area of pesticide effects on cardiac biochemistry especially as related to their influence on calcium regulation.