IMMUNOLOGY AND DISEASES OF THE GASTROINTESTINAL TRACT

免疫学和胃肠道疾病

• 批准号: 3225113
• 负责人: JAMES E. MC GUIGAN
• 金额: $ 17.74万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-01-01 至 1989-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3225113
• 关键词: Zollinger Ellison syndrome; adrenergic agents; biopsy; calcium; calcium metabolism; carbachol; catecholamines; cholinergic agents; cyclic AMP; duodenal ulcer; electron microscopy; endocrine pharmacology; female reproductive system disorder; gastric inhibitory peptide; gastric mucosa; gastrins; gastrointestinal hormones; gastrointestinal pharmacology; histochemistry /cytochemistry; hormone regulation /control mechanism; hormone related neoplasm /cancer; human tissue; immunochemistry; immunofluorescence technique; intestinal mucosa; organ culture; peptide hormone biosynthesis; propranolol; radioimmunoassay; radiotracer; secretin; secretion; somatostatin; theophylline; vasoactive intestinal peptide

This research proposal is directed to the study of gastrointestinal regula- tory peptides, with particular emphasis on gastrin, which plays a crucial role in the regulation of gastric acid secretion. Studies will utilize a variety of immunological and immunochemical techniques to examine content, release, and interrelationships of certain gastrointestinal regulatory peptides utilizing a variety of methods, including organ culture, animal models and in studies in man. Studies are designed to identify factors which participate in regulation of gastrin release, including somatostatin and gastrin releaseing peptide, a substance believed to be the mammalian equivalent of bombesin. Pulse-chase experiments will be formed with antral mucosa and gastrinoma tissue to identify potential precursor-product relationships of multiple molecular species of gastrin. In vitro and in vivo studies will be performed utilizing antibodies to various regulatory peptides, including antibodies to somatostatin, gastrin releasing peptide, and gastrin to analyze the potential role played by these peptides and their effects in mediation of other peptide effects. The effects of various nutrients on promotion of gastrointestinal regulatory peptide release and physiological effects will be determined, as will assessment of the nature of the molecular species of regulatory peptides released by different forms of feeding. Patients with duodenal ulcer often exhibit increased gastric acid secretion and a significant portion of these patients demonstrate increased gastrin release in response to feeding. Studies will be performed to examine the relationship between somatostatin and gastrin in antral mucosa of duodenal ulcer patients compared with non-ulcer subjects, and the potential role of somatostatin and other regulatory peptides in modifying gastrin release and/or gastric acid secretion. Antibodies with precise specificity for limited peptide sequence of gastrin molecules will be used to define gastrin forms in tissues and in the circulation, released in response to stimulation. Region-specific antibodies will be used to identify and discriminate among molecular forms of CCK and gastrin.

本研究旨在研究胃肠调节的作用， tory肽，特别强调胃泌素，它起着关键的作用， 调节胃酸分泌的作用。 研究将利用 各种免疫学和免疫化学技术来检测含量， 释放，以及某些胃肠调节的相互关系， 利用多种方法，包括器官培养、动物培养、 模型和人体研究。研究旨在确定 参与调节胃泌素的释放，包括生长抑素 和胃泌素释放肽，一种被认为是哺乳动物 相当于蛙皮素。 脉冲追逐实验将形成与胃窦 粘膜和胃泌素瘤组织，以鉴定潜在的促胃液素产物 胃泌素的多个分子种类的关系。 体外和 体内研究将利用针对各种调节因子的抗体进行 肽，包括生长抑素，胃泌素释放肽， 和胃泌素来分析这些肽所起的潜在作用， 它们在介导其它肽作用中的作用。 的影响 多种营养素对胃肠调节肽的促进作用 释放和生理效应将被确定， 释放的调节肽的分子种类的性质 不同的喂养方式。 十二指肠溃疡患者常表现为 胃酸分泌增加，其中很大一部分 患者表现出响应于进食的增加的胃泌素释放。 将进行研究，以检查生长抑素与 与对照组比较， 非溃疡受试者，以及生长抑素和其他 调节胃泌素释放和/或胃酸的调节肽 分泌物 对有限肽具有精确特异性的抗体 胃泌素分子序列将用于定义胃泌素形式， 组织和循环中，响应刺激而释放。 区域特异性抗体将用于识别和区分 CCK和胃泌素的分子形式。