Determining the mechanism of septin-mediated plant infection by the rice blast fungus Magnaporthe oryzae

确定稻瘟病菌 Magnaporthe oryzae 败血症介导的植物感染机制

• 批准号: BB/N009959/2
• 负责人: Nicholas Talbot
• 金额: $ 22.44万
• 依托单位: University of East Anglia
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FN009959%2F2
• 关键词: Determining; mechanism; septin; mediated; plant

The aim of this project is to understand how rice plants succumb to a very serious disease called rice blast. Each year, rice blast disease destroys up to 30% of the global rice harvest and causes serious epidemics in Sub-Saharan Africa, South-East Asia and South America. It is therefore a continuing threat to global food security. Rice blast is caused by a fungus called Magnaporthe oryzae and this project aims to determine how the fungus infects rice plants. The rice blast fungus produces a specialised infection structure called an appressorium, which generates enormous pressure (up to 8MPa, or 40 times the pressure of a car tyre) and to apply physical force at the leaf surface to puncture the plant cuticle. In this way the fungus can invade leaf tissue and cause disease. We aim to investigate how pressure inside the appressorium is translated into physical force at the base of the infection cell. We have discovered that a group of protein called septins, are essential for the appressorium to puncture the rice cuticle. Their role is to re-model the cell's internal cytoskeleton so it applies force at the leaf surface and forms a penetration peg that enter the leaf. This project will investigate how septins assemble at the base of the appressorium and how this region of the cell becomes specialised to secrete proteins into plant cells, how the appressorium develops a penetration peg, and how the fungus then rapidly invades the leaf. We will characterise how septin assembly is regulated and, in particular, how the fungus is able to monitor the turgor pressure within the appressorium and determine the optimal point (or trigger) for penetration peg development. We will also determine how this process is regulated in concert with the cell division cycle of the fungus, allowing the plant infection process to be controlled effectively.When considered together, the objectives of this research project will provide new insight into the biology of plant infection by one of the most important crop diseases in the world today. This information will be used to inform new disease control strategies that are urgently required. In addition to the global significance of rice blast, knowledge gained from the project will also be of value to UK agriculture because many of the most serious diseases that affect our major cereal crops, barley and wheat, share a similar infection mechanism. Disease control strategies emerging from this work are therefore likely to be of broad spectrum for the most important cereal diseases, such as rusts and powdery mildews in addition to rice blast.

这个项目的目的是了解水稻如何屈服于一种非常严重的疾病，称为稻瘟病。每年，稻瘟病摧毁了全球30%的水稻收成，并在撒哈拉以南非洲、东南亚和南美洲造成严重流行。因此，这是对全球粮食安全的持续威胁。稻瘟病是由一种名为Magnaporthe的真菌引起的，该项目旨在确定真菌如何感染水稻。稻瘟病真菌产生一种称为附着胞的特殊感染结构，它产生巨大的压力（高达8 MPa，或汽车轮胎压力的40倍），并在叶片表面施加物理力以刺穿植物角质层。通过这种方式，真菌可以侵入叶组织并引起疾病。我们的目的是研究附着胞内的压力如何转化为感染细胞基部的物理力。我们已经发现一组称为septins的蛋白质是附着胞刺穿水稻角质层所必需的。它们的作用是重新塑造细胞的内部细胞骨架，使其在叶片表面施加力，并形成一个进入叶片的穿透钉。该项目将研究隔膜如何在附着胞的基部组装，以及细胞的这一区域如何专门分泌蛋白质进入植物细胞，附着胞如何形成渗透钉，以及真菌如何快速侵入叶片。我们将探讨隔蛋白组装是如何调节的，特别是，真菌是如何能够监测附着胞内的膨压，并确定最佳点（或触发器）的渗透钉发展。我们还将确定这一过程是如何与真菌的细胞分裂周期相协调的，从而使植物感染过程得到有效控制。综合考虑，本研究项目的目标将为当今世界上最重要的作物病害之一的植物感染生物学提供新的见解。这些信息将用于为迫切需要的新疾病控制战略提供信息。除了稻瘟病的全球意义外，从该项目中获得的知识也将对英国农业有价值，因为影响我们主要谷物作物大麦和小麦的许多最严重的疾病都有类似的感染机制。因此，从这项工作中出现的疾病控制策略可能是最重要的谷物疾病，如锈病和白粉病除了稻瘟病广谱。

项目成果

CRISPR-Cas9 ribonucleoprotein-mediated co-editing and counterselection in the rice blast fungus.

• DOI: 10.1038/s41598-018-32702-w
• 发表时间: 2018-09-25
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Foster AJ;Martin-Urdiroz M;Yan X;Wright HS;Soanes DM;Talbot NJ
• 通讯作者: Talbot NJ

Clathrin-mediated Endocytosis Facilitates Internalization of Magnaporthe oryzae Effectors into Rice Cells

• DOI: 10.1101/2021.12.28.474284
• 发表时间: 2021-12
• 期刊: bioRxiv
• 作者: Ely Oliveira-Garcia;Tej Man Tamang;Jungeun Park;Melinda Dalby;Magdalena Martin-Urdiroz;Clara Rodriguez Herrero;Annie Vu;Sunghun Park;N. Talbot;B. Valent

Chitosan inhibits septin-mediated plant infection by the rice blast fungus Magnaporthe oryzae in a Protein Kinase C and Nox1 NADPH oxidase-dependent manner

• DOI: 10.1101/2020.05.15.098657
• 发表时间: 2020-05
• 期刊: bioRxiv
• 作者: Federico López-Moya;Magdalena Martin-Urdiroz;Míriam Osés-Ruiz;M. Fricker;George R. Littlejohn;L. V. Lopez-Llorca

A hierarchical transcriptional network controls appressorium-mediated plant infection by the rice blast fungus Magnaporthe oryzae

• DOI: 10.1101/2020.02.05.936203
• 发表时间: 2020-02
• 期刊: bioRxiv
• 作者: Míriam Osés-Ruiz;Magdalena Martin-Urdiroz;D. Soanes;M. J. Kershaw;Neftaly Cruz-Mireles;G. Valdovinos-Ponce;Camilla Molinari;George R. Littlejohn;P. Derbyshire;Frank L. H. Menke;B. Valent;N. Talbot