Development of NanoKick Bioreactor

NanoKick生物反应器的开发

• 批准号: BB/N012690/1
• 负责人: Matthew Dalby
• 金额: $ 25.07万
• 依托单位: University of Glasgow
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2016
• 资助国家: 英国
• 起止时间: 2016 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FN012690%2F1
• 关键词: Development; NanoKick; Bioreactor

Bone graft is the most transplanted tissue after blood. It is in short supply and demand is increasing with the aging population. Taking graft from a patient to transplant back to the patient is possible but results in morbidity of the donor site (normally the pelvis). We have developed a novel bioreactor, called Nanokick, which can be used to efficiently and effectively convert mesenchymal stem cells (MSCs - the bones natural stem cell supply) into osteoblasts (bone building cells). It works by supplying tiny kicks to cells. Our approach will provide technology that enables provision of 3D living bone derived from a patients own cells that produces effective repair. Areas this would be useful would include all bone reconstructions, spinal fusion and joint replacement. These in no need for chemicals and associated side effects (e.g. widely used bone morphogenic protein is now associated with adverse effects). In this work we will develop our technology towards a commercial product.

骨移植物是继血液之后移植最多的组织。随着人口老龄化，供应短缺，需求增加。从患者身上取下移植物移植回患者体内是可能的，但会导致供体部位（通常是骨盆）发病。我们开发了一种新型生物反应器，称为Nanokick，可用于有效地将间充质干细胞（MSC-骨骼天然干细胞供应）转化为成骨细胞（骨骼构建细胞）。它的工作原理是向细胞提供微小的刺激。我们的方法将提供一种技术，能够提供来自患者自身细胞的3D活骨，从而产生有效的修复。这将是有用的领域将包括所有的骨重建，脊柱融合和关节置换。这些不需要化学品和相关的副作用（例如，广泛使用的骨形态发生蛋白现在与不良反应有关）。在这项工作中，我们将把我们的技术发展成为商业产品。

项目成果

Design, construction and characterisation of a novel nanovibrational bioreactor and cultureware for osteogenesis

用于成骨的新型纳米振动生物反应器和培养器皿的设计、构建和表征

• DOI: 10.1101/543660
• 发表时间: 2019
• 作者: Campsie P

The use of nanovibration to discover specific and potent bioactive metabolites that stimulate osteogenic differentiation in mesenchymal stem cells.

• DOI: 10.1126/sciadv.abb7921
• 发表时间: 2021-03
• 期刊: Science advances
• 影响因子: 13.6
• 作者: Hodgkinson T;Tsimbouri PM;Llopis-Hernandez V;Campsie P;Scurr D;Childs PG;Phillips D;Donnelly S;Wells JA;O'Brien FJ;Salmeron-Sanchez M;Burgess K;Alexander M;Vassalli M;Oreffo ROC;Reid S;France DJ;Dalby MJ
• 通讯作者: Dalby MJ

Nanovibrational stimulation inhibits osteoclastogenesis and enhances osteogenesis in co-cultures.

• DOI: 10.1038/s41598-021-02139-9
• 发表时间: 2021-11-23
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Kennedy JW;Tsimbouri PM;Campsie P;Sood S;Childs PG;Reid S;Young PS;Meek DRM;Goodyear CS;Dalby MJ
• 通讯作者: Dalby MJ

Engineered microenvironments for synergistic VEGF - Integrin signalling during vascularization.

• DOI: 10.1016/j.biomaterials.2017.02.024
• 发表时间: 2017-05
• 期刊: Biomaterials
• 影响因子: 14
• 作者: Moulisová V;Gonzalez-García C;Cantini M;Rodrigo-Navarro A;Weaver J;Costell M;Sabater I Serra R;Dalby MJ;García AJ;Salmerón-Sánchez M
• 通讯作者: Salmerón-Sánchez M

Hurdles to uptake of mesenchymal stem cells and their progenitors in therapeutic products.

• DOI: 10.1042/bcj20190382
• 发表时间: 2020-09-18
• 期刊: The Biochemical journal
• 作者: Childs PG;Reid S;Salmeron-Sanchez M;Dalby MJ
• 通讯作者: Dalby MJ