CYTOCHEMISTRY OF CENTRAL AUDITORY PATHWAYS

中枢听觉通路的细胞化学

• 批准号: 3217413
• 负责人: RICHARD L SAINT MARIE
• 金额: $ 13.07万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-03-01 至 1993-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3217413
• 关键词: afferent nerve; aspartate; auditory feedback; auditory pathways; autoradiography; axon; binaural hearing; cats; cell type; chinchilla; cues; efferent nerve; gamma aminobutyrate; glutamates; glycine; immunochemistry; inferior colliculus; neural information processing; neuronal transport; neurotransmitters; sound frequency; synapses

Binaural processing is important because it allows organisms to localize sounds in their environment. Evidence of this processing can be found at early levels of central auditory pathways in mammals, including nuclei of the superior olive, lateral lemniscus, and inferior colliculus. Neurons in these pathways use interaural time and level differences (ITD's and ILD'S) to compute the source of a sound. Part of this processing involves the interaction of excitatory inputs driven by one ear and inhibitory inputs driven by the other ear. In this project we will investigate which neurons in the auditory pathways provide inhibitory inputs for binaural processing, and how these inputs converge with excitatory inputs at different levels of the auditory pathway. We will relate these findings to the processing of ITD's and ILD's in nuclei of the superior olive, lateral lemniscus, and inferior colliculus. To do this, immunocytochemistry and transmitter-specific uptake will be used to identify which neurons in the nuclei of the superior olive, lateral lemniscus, and inferior colliculus may use inhibitory or excitatory transmitters. These procedures will be combined with retrograde transport of exogenously injected tracers to identify inhibitory or excitatory neurons with ascending projections. Moreover, anterograde tracing of the afferent inputs to these projection neurons will be combined with retrograde transport and immunocytochemistry. In this way we will define the chains of neurons and their inhibitory connections which are likely to be involved in the binaural processing of ITD's and ILD'S.

双耳处理很重要，因为它允许生物体定位