Understanding the functional and genomic architecture of the rumen microbiome affecting performance traits in bovines

了解影响牛性能特征的瘤胃微生物组的功能和基因组结构

• 批准号: BB/N01720X/1
• 负责人: Rainer Roehe
• 金额: $ 39.03万
• 依托单位: Scotland's Rural College
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2016
• 资助国家: 英国
• 起止时间: 2016 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FN01720X%2F1
• 关键词: Understanding; functional; genomic; architecture; rumen

By 2050, the human population will grow to over 9 billion people, and in the same time frame, global meat production is set to increase by 73%. There is a need to increase the efficiency and sustainability of animal production, reduce waste in the food chain and ensure safe and nutritious diets in order to address this challenge. Rumen microbes confers a unique ability to convert human inedible high-fibre forage into nutrients the animal can absorb to produce high-quality proteins as meat and milk. However, intensive food production puts a strain on the environment, and there is a need to produce more food ethically and in a way that does not harm the environment. The project addresses these challenges by unravelling the functional and genomic architecture of the ruminal microbiome affecting performance traits of cattle. This information will be used to identify fundamental associations between the microbiome or its genes with animal performance traits and methane emissions. In this study we will sequence all microbial genomes - the metagenome - to describe the composition of the microbial community and its functional genes. The analysis will be based on a unique dataset of 288 experimental beef cattle, with rumen DNA samples and a large array of performance information (e.g. feed conversion efficiency, growth, body composition and meat quality) available. These data are structured by breeds and sire progeny groups to estimate the animal host genetic effects on the microbiome and microbial genes. The experimental data have been the basis of numerous publications in which it was shown that at the animal performance level, and for methane emissions, there are large differences between breeds, sire progeny groups and diets. Preliminary analysis for 8 of these animals suggests that there is a link between the abundance of the microbial community or microbial genes and animal performance traits and methane emissions. However, to understand the function and genomic architecture of the ruminal microbiome, analysis of the full sample set is necessary. Algorithms will be developed to predict animal performance, e.g. feed conversion efficiency and methane emissions from the abundance of the microbial community and genes. These high value, but costly-to-measure traits could then be predicted by analysing the rumen microbiome (sampled via stomach tube on live animals or in the abattoir). However, to verify the associations between the rumen microbiome and performance traits, we need basic knowledge about the functional and genomic architecture of the microbiome. Additionally, microbial biomarkers to predict e.g. feed conversion efficiency could be identified. Due to the unique structure of the data in sire progeny groups and diets, we will be able to predict the host genetic and nutritional effect on the microbial community and microbial genes. This structure can also be used in the network analysis to identify animal genetic effects on the functional and genomic architecture of the microbiome. The project will provide unprecedented new knowledge of the genomic and functional architecture of the microbiome and its impact on performance traits and methane emissions as well as the interaction with animal genetics and nutrition. We will compare the functional and genetic architecture of the microbiome in beef cattle with that of other species to provide insights about the microbiome of different species, in particular humans. By understanding host genetic effects on the rumen microbiota and associations with body composition, we expect to provide new insights for human personalised medicine approaches to reduce obesity.

到2050年，全球人口将超过90亿，在同一时间段内，全球肉类产量将增长73%。为了应对这一挑战，需要提高畜牧生产的效率和可持续性，减少食物链中的浪费，并确保安全和营养的饮食。瘤胃微生物赋予了一种独特的能力，将人类不可食用的高纤维饲料转化为动物可以吸收的营养物质，以生产高质量的蛋白质，如肉和奶。然而，集约化的粮食生产对环境造成了压力，需要以合乎道德的方式生产更多的粮食，而不损害环境。该项目通过揭示影响牛的性能性状的瘤胃微生物组的功能和基因组结构来解决这些挑战。这些信息将用于确定微生物组或其基因与动物性能性状和甲烷排放之间的基本关联。在这项研究中，我们将测序所有微生物基因组-宏基因组-来描述微生物群落及其功能基因的组成。该分析将基于288头实验肉牛的独特数据集，提供瘤胃DNA样本和大量性能信息（例如饲料转化效率，生长，身体组成和肉质）。这些数据按品种和父系后代组进行结构化，以估计动物宿主对微生物组和微生物基因的遗传效应。实验数据已经成为许多出版物的基础，其中表明，在动物性能水平和甲烷排放量方面，品种、父系后代组和饮食之间存在很大差异。对其中8只动物的初步分析表明，微生物群落或微生物基因的丰度与动物性能特征和甲烷排放量之间存在联系。然而，为了了解瘤胃微生物组的功能和基因组结构，需要分析整个样品集。将开发算法来预测动物的性能，例如饲料转化效率和微生物群落和基因丰度的甲烷排放。这些高价值但测量成本高的特性可以通过分析瘤胃微生物组（通过活体动物或屠宰场中的胃管采样）来预测。然而，为了验证瘤胃微生物组与生产性状之间的关联，我们需要有关微生物组功能和基因组结构的基本知识。此外，可以鉴定用于预测例如饲料转化效率的微生物生物标志物。由于父系后代群体和饮食数据的独特结构，我们将能够预测宿主遗传和营养对微生物群落和微生物基因的影响。这种结构也可以用于网络分析，以确定动物遗传对微生物组的功能和基因组结构的影响。该项目将提供有关微生物组的基因组和功能结构及其对性能性状和甲烷排放的影响以及与动物遗传学和营养的相互作用的前所未有的新知识。我们将比较肉牛微生物组的功能和遗传结构与其他物种的微生物组，以提供有关不同物种，特别是人类微生物组的见解。通过了解宿主基因对瘤胃微生物群的影响以及与身体组成的关系，我们希望为人类减少肥胖的个性化医学方法提供新的见解。

项目成果

Invited review: Novel methods and perspectives for modulating the rumen microbiome through selective breeding as a means to improve complex traits: Implications for methane emissions in cattle

特邀评论：通过选择性育种调节瘤胃微生物组作为改善复杂性状的一种手段的新方法和观点：对牛甲烷排放的影响

• DOI: 10.1016/j.livsci.2023.105171
• 发表时间: 2023
• 期刊: Livestock Science
• 影响因子: 1.8
• 作者: González-Recio O

A network analysis of methane and feed conversion genes in the rumen microbial community

• DOI: 10.1109/bibm.2016.7822741
• 发表时间: 2016-12
• 期刊: 2016 IEEE International Conference on Bioinformatics and Biomedicine (BIBM)
• 作者: Fiona Browne;Haiying Wang;Huiru Zheng;R. Roehe;R. Dewhurst;P. Walsh

The rumen microbiome as a reservoir of antimicrobial resistance and pathogenicity genes is directly affected by diet in beef cattle.

• DOI: 10.1186/s40168-017-0378-z
• 发表时间: 2017-12-11
• 期刊: Microbiome
• 影响因子: 15.5
• 作者: Auffret MD;Dewhurst RJ;Duthie CA;Rooke JA;John Wallace R;Freeman TC;Stewart R;Watson M;Roehe R
• 通讯作者: Roehe R

Identification, Comparison, and Validation of Robust Rumen Microbial Biomarkers for Methane Emissions Using Diverse Bos Taurus Breeds and Basal Diets.

• DOI: 10.3389/fmicb.2017.02642
• 发表时间: 2017
• 期刊: Frontiers in microbiology
• 影响因子: 5.2
• 作者: Auffret MD;Stewart R;Dewhurst RJ;Duthie CA;Rooke JA;Wallace RJ;Freeman TC;Snelling TJ;Watson M;Roehe R
• 通讯作者: Roehe R

Additional file 1: Figure S1. of The rumen microbiome as a reservoir of antimicrobial resistance and pathogenicity genes is directly affected by diet in beef cattle

附加文件 1：图 S1。

• DOI: 10.6084/m9.figshare.5691460
• 发表时间: 2017
• 作者: Auffret M