NEUTROPHIL FUNCTION IN PERIODONTAL DISEASE

伴放线放线杆菌（Aa）和拟杆菌 牙龈炎（Bg）与某些形式的牙龈炎的病因学有关。 牙周病 有人认为，中性粒细胞 在防御这些建立中起着主要作用 牙周病病原体 本报告的总体目标 建议的工作是定义参与的PMN的机制， 预防牙周病的方法， 牙周病患者的功能缺陷 疾病 已经确定， 局限性青少年牙周炎（LJP）有定量的 趋化性缺陷 LJP PMN的初步研究表明 它们能够正常吞噬Aa;然而，它们不能 来杀死这些有机体。 因此，这些研究将审查 LJP PMN以确定导致的潜在功能障碍 无法杀死安 首先将确定是否 功能障碍是特定的或普遍的缺陷，在杀死所有 通过检查LJP PMN杀死非细菌的能力， 只有Aa，还有Bg、金黄色葡萄球菌、大肠杆菌， 变形链球菌。 LJP PMN的杀菌活性 将与健康对照组和患者组进行比较， 慢性成人或难治性牙周炎， 不存在患者和对照血清和唾液。 最近的研究 表明存在不同的PMN颗粒亚群， 构成功能上不同的种群。 一种质或 这些颗粒子集中的一个或多个中的质量缺陷可以 导致无法杀死特定的微生物或一组 微生物 用等密度线获得的颗粒轮廓 患者和对照PMN组分的梯度离心 将使用选定颗粒的生化分析进行比较 标记。 此外，还测定了所述组合物的颗粒含量和上清液。 将在刺激后检查各种细胞群 调理试验微生物或特异性促分泌物。 是 进一步提出研究1gA-Fc的功能能力， 中性粒细胞受体阳性亚群的变化及 血清和分泌性抗体。 的表面特性 1gA+和1gA-PMN将使用其他配体表征， 通过直接结合测定的膜结构的探针。 研究 这些患者群体提供了一个独特的机会， 中性粒细胞颗粒和酶亚群的功能作用， 细胞亚群与对特定 牙周病病原体

Actinobacillus actinomycetemcomitans (Aa) and Bacteroides gingivalis (Bg) have implicated in the etiology of certain forms of periodontal disease. It has been suggested that the neutrophil plays a primary role in the defense against establishment of these purported periodontopathogens. The overall objective of this proposed work is to define the mechanisms of the PMN involved in the defense against periodontal diseases by examining and defining the functional defects of patients with periodontal disease. It is well established that the PMN of patients with localized juvenile periodontitis (LJP) have quantitative chemotactic defects. Preliminary studies with LJP PMN suggest they are capable of normal phagocytoses of Aa; however, they fail to kill these organisms. These studies will, therefore, examine the LJP PMN to determine the underlying dysfunction that results in this inability to kill Aa. It will first be determined if this dysfunction is specific or a generalized deficiency in killing of all microorganisms by examining LJP PMN of their ability to kill not only Aa, but also Bg, Staphylococcus aureus, Escherichia coli, and Streptococcus mutans. The bactericidal activity of the LJP PMN will be compared with that of healthy controls, and of patients with chronic adult or refractory periodontitis in the presence and absence of patient and control sera and saliva. Recent studies indicate that there are distinct subsets of PMN granules that may constitute functionally distinct populations. A quantitative or qualitative defect in one or more of these granule subsets may result in an inability to kill a specific microoganisms or group of microoganisms. The granule profiles obtained by isopycnic gradient centrifugation of fractions of patient and control PMN will be compared using biochemical analyses for selected granule markers. In addition, the granule content and supernatants of the various cell populations will be examined following stimulation with opsonized test organisms or specific secretogogues. It is further proposed to study the functional abilities of 1gA-Fc receptor positive subpopulation of PMN and their influence by serum and secretory antibodies. The surface characteristics of 1gA+ and 1gA- PMNs will be characterized using other ligands as probes of membrane structure by direct binding assays. A study of these patient populations offers a unique opportunity to dissect the functional roles of subsets of PMN granules and enzymes and subset populations of cells in relation to susceptibility to specific periodontopathogens.