NEUTROPHIL FUNCTION IN PERIODONTAL DISEASE

伴生放线杆菌(AA)和类杆菌 牙周炎(BG)与某些形式的牙周炎的病因学有关。 牙周病。有人认为，中性粒细胞 在防御这些建立的过程中发挥主要作用 据称是牙周病原体。这样做的总体目标是 建议的工作是定义PMN所涉及的机制 通过检查和检查预防牙周病 牙周病患者功能缺陷的界定 疾病。已有的研究表明，慢性阻塞性肺疾病患者的中性粒细胞 局限性青少年牙周炎(LJP)有定量 趋化性缺陷。对LJP PMN的初步研究表明 它们能够正常吞噬AA；然而，它们失败了 来杀死这些生物。因此，这些研究将审查 LJP PMN以确定导致的潜在功能障碍 在这种无法杀死AA的情况下。首先要确定的是，这个 功能障碍是杀死所有人的一种特殊的或普遍的缺陷 通过检测LJP PMN对微生物的杀灭能力 不仅有AA，而且还有BG、金黄色葡萄球菌、大肠杆菌和 变形链球菌。LJP中性粒细胞的杀菌活性 将与健康对照组和患者进行比较 患有慢性成人或难治性牙周炎 没有患者和对照血清和唾液。最新研究 表明存在PMN颗粒不同子集，其可以 构成功能不同的种群。数量或 这些颗粒子集中的一个或多个中的质量缺陷可以 导致不能杀死特定微生物或一组 微生物。等密度梯度法测得的颗粒轮廓 患者和对照中性粒细胞部分的梯度离心法 将使用生化分析对选定的颗粒进行比较 记号笔。此外，还测定了颗粒含量和上清液。 各种细胞群将在刺激后进行检查 用调光的测试有机体或特定的分泌物。它是 进一步建议研究1gA-Fc的功能能力 中性粒细胞受体阳性亚群及其对其影响 血清和分泌性抗体。它的表面特征 1gA+和1gA-PMN将使用以下其他配体进行表征 膜结构的直接结合法探头。一项研究 为这些患者群体提供了一个独特的机会 中性粒细胞颗粒和酶亚群的功能作用 细胞亚群与特定病毒易感性的关系 牙周病原体。

Actinobacillus actinomycetemcomitans (Aa) and Bacteroides gingivalis (Bg) have implicated in the etiology of certain forms of periodontal disease. It has been suggested that the neutrophil plays a primary role in the defense against establishment of these purported periodontopathogens. The overall objective of this proposed work is to define the mechanisms of the PMN involved in the defense against periodontal diseases by examining and defining the functional defects of patients with periodontal disease. It is well established that the PMN of patients with localized juvenile periodontitis (LJP) have quantitative chemotactic defects. Preliminary studies with LJP PMN suggest they are capable of normal phagocytoses of Aa; however, they fail to kill these organisms. These studies will, therefore, examine the LJP PMN to determine the underlying dysfunction that results in this inability to kill Aa. It will first be determined if this dysfunction is specific or a generalized deficiency in killing of all microorganisms by examining LJP PMN of their ability to kill not only Aa, but also Bg, Staphylococcus aureus, Escherichia coli, and Streptococcus mutans. The bactericidal activity of the LJP PMN will be compared with that of healthy controls, and of patients with chronic adult or refractory periodontitis in the presence and absence of patient and control sera and saliva. Recent studies indicate that there are distinct subsets of PMN granules that may constitute functionally distinct populations. A quantitative or qualitative defect in one or more of these granule subsets may result in an inability to kill a specific microoganisms or group of microoganisms. The granule profiles obtained by isopycnic gradient centrifugation of fractions of patient and control PMN will be compared using biochemical analyses for selected granule markers. In addition, the granule content and supernatants of the various cell populations will be examined following stimulation with opsonized test organisms or specific secretogogues. It is further proposed to study the functional abilities of 1gA-Fc receptor positive subpopulation of PMN and their influence by serum and secretory antibodies. The surface characteristics of 1gA+ and 1gA- PMNs will be characterized using other ligands as probes of membrane structure by direct binding assays. A study of these patient populations offers a unique opportunity to dissect the functional roles of subsets of PMN granules and enzymes and subset populations of cells in relation to susceptibility to specific periodontopathogens.