CELL SURFACE INTERACTIONS IN ORAL PLAQUE BACTERIA

口腔菌斑细菌中细胞表面的相互作用

• 批准号: 3220093
• 负责人: JOSEPH M. DIRIENZO
• 金额: $ 11.56万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-09-01 至 1990-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3220093
• 关键词: Fusobacterium; Streptococcus sanguis; anaerobic bacteria; dental plaque; gram negative bacteria; gram positive bacteria; immunochemistry; membrane activity; oral bacteria; periodontium disorder; physical chemical interaction

Corncobs are distinct morphological units formed by the ordered coaggregation of a filamentous microorganism and streptococci. At saturating numbers the adherent streptococci coat the surface of the filament so that the complex resembles an ear of corn. These corncob structures have been observed in developing or maturing human dental plaque and it has been suggested that corncob formation represents a connecting link between supra- and subgingival plaque. Our earlier work has shown that Fusobacterium nucleatum,an anaerobic gram-negative bacterium thought to be involved in some forms of periodontal disease, has the ability to form corncobs, in vitro, with strains of Streptococcus sanguis. One objectives are to characterize the previously identified corncob-receptor of F. nucleatum ATCC 10953, construct immunological and genetic probes for the detection and analysis of this receptor, and develop strategies designed to inhibit or block the corncob reaction in vitro. An active tryptic polypeptide of the proposed corncob receptor will be isolated on a preparative scale by immunoaffinity chromatography using monospecific polyclonal antibodies. The polypeptide will be characterized using classical biochemical procedures and the amino terminal portion of the molecule will be sequenced. Information derived from this sequence will be applied to the production of DNA hybridization probes which will be used for both the detection of the cloned structural gene, in recombinant plasmids, and the determination of the distribution of this specific corncob receptor in oral clinical isolates of Fusobacterium nucleatum. The anti-receptor antibodies and selected peptides, derived from the proteolytic digestion of the receptor protein will be evaluated, in competition and direct binding studies, for the ability to prevent corncob formation in vitro. Development and application of immunological and genetic probes should greatly enhance our ability to define the complex intergeneric coaggregation mechanisms that occur in the oral cavity. Furthermore, the results should lead to the production bf reagents which could be used to prevent the maturation of plaque and reduce the incidence of one possible inflammatory mechanism associated with the progression of gingivitis and periodontitis.

玉米棒是由有序的 丝状微生物和链球菌的共聚集。 在 附着的链球菌覆盖在 这样复合体就像一个玉米穗。 这些 在发育或成熟过程中观察到玉米芯结构 人类的牙菌斑，并且有人认为， 形成代表了一个连接链接之间的supra和 龈下菌斑 我们早期的研究表明梭杆菌 nucleatum，一种厌氧革兰氏阴性细菌，被认为是 牙周病的症状有哪些？ 形成玉米芯，在体外，与血链球菌菌株。 一个目标是描述以前确定的 F.核质ATCC 10953，构建体 免疫学和遗传学探针，用于检测和分析 这种受体，并开发旨在抑制或阻断 体外玉米芯反应。 一种活性胰蛋白酶多肽， 所提出的玉米芯受体将在制备型分离器上分离， 采用单特异性免疫亲和色谱法测定规模 多克隆抗体。 所述多肽将使用以下来表征： 经典的生物化学程序和氨基末端部分 分子将被测序。 信息来源于此 序列将应用于DNA杂交的生产 探针将用于检测克隆的 重组质粒中的结构基因，以及 这种特异性玉米芯受体在口腔临床中的分布 具核梭杆菌的分离物。 抗受体抗体 和选择的肽，来源于蛋白水解消化的 受体蛋白将在竞争和直接 结合研究，以防止玉米穗轴形成的能力， 体外 免疫遗传学的发展与应用 探测器应该能大大增强我们对复杂的 发生在口腔中的属间共聚集机制 腔 此外，结果应导致生产BF 可用于防止菌斑成熟的试剂 并减少一种可能的炎症机制的发生率 与牙龈炎和牙周炎的进展相关。