BACTERIAL PROTEINASES IN PERIODONTAL DISEASE

牙周疾病中的细菌蛋白酶

• 批准号: 3223524
• 负责人: James Travis
• 金额: $ 13.52万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-01 至 1996-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3223524
• 关键词: Bacteroides gingivalis; blood proteins; collagen; elastin; endopeptidases; enzyme induction /repression; enzyme mechanism; enzyme structure; enzyme substrate; enzyme substrate analog; fibronectins; human tissue; laboratory rabbit; lysozyme; neutrophil; periodontitis; protease inhibitor; protein purification

The development of periodontal disease in adults is believed to be associated with the localized secretion of proteinases from the organism Porphyromonas qingivalis into the dento-gingival cavity. These enzymes can degrade a host of important proteins which include not only those involved in connective tissue structure (e.g., collagen) but also regulato factors such as components, proteinase inhibitors, and bactericidal proteins. Since it is likely that the presence of P. gingivalis causes the recruitment of neutrophils to the dento-gingival cavity and the release of phagocytic proteinases which augment abnormal connective tissue turn-over, the inactivation of regulatory proteins becomes even more important. In order to study the potential dual role of P. gingivalis proteinases more closely, the specific aims of this project are dedicated to 1) their isolation and characterization, 2) determination of their specificity, particularly with regard to the hydrolysis of synthetic substrates and naturally occurring proteins, 3) evaluation of their ability to degrade both inflammatory and anti-inflammatory proteins, and 4) measurement of the effect of naturally occurring proteinase inhibitors (mammalian and bacteria derived) as potential regulators. The long term goals of this proposal are to evaluate the contribution of P. gingivalis proteinases to the development of periodontal disease, determine the structure of these enzymes, and produce inhibitors which can regulate their activity in vivo.

牙周病的发病原因有哪些？ 与生物体蛋白酶的局部分泌有关 牙龈卟啉单胞菌进入牙-龈腔。 这些酶 可以降解许多重要的蛋白质， 参与结缔组织结构（例如，胶原蛋白），而且 调节因子如组分、蛋白酶抑制剂，和 杀菌蛋白 由于P. 牙龈炎引起中性粒细胞向牙-牙龈组织的募集 腔和吞噬蛋白酶的释放， 结缔组织翻转，调节蛋白失活 变得更加重要 为了研究潜在的双重作用 牙龈卟啉单胞菌蛋白酶更密切，这一特定的目的， 项目致力于1）它们的分离和表征，2） 确定其特异性，特别是关于 合成底物和天然存在的蛋白质的水解，3） 评价它们降解炎症和炎症的能力， 抗炎蛋白，和4）测量 天然存在的蛋白酶抑制剂（哺乳动物和细菌 作为潜在的监管机构。本提案的长期目标 是为了评估牙龈卟啉单胞菌蛋白酶的贡献， 牙周病的发展，确定这些结构 酶，并产生抑制剂，可以调节它们的活性， vivo.