PROTEIN INHIBITORS OF CALCIUM PHOSPHATE PRECIPITATION

人类唾液中的碱性钙高度饱和。 磷酸盐。然而，从唾液和唾液中沉淀这些盐 磷酸钙在牙釉质上不会发生晶体生长 正常的生理状态。这种出乎意料的稳定性是由于 他汀类两种唾液磷蛋白的抑制活性 和酸性富含脯氨酸的磷酸蛋白(PRP)。在生理上 条件下，他汀类药物抑制自发沉淀和晶体生长 磷酸钙，而PRP则是晶体的有效抑制物 通过吸附在钙磷酸盐矿物表面而生长，如 牙釉质矿物。这些活动在以下方面具有生物学意义 它们的作用是提供一个过饱和的，但稳定的，保护性的 牙釉质的修复环境，这对牙釉质 牙齿的完整性。 这个项目的基本目标是将 这些抑制物对它们在口腔中的功能有影响。最严谨的 解决这个问题的方法是通过化学合成这些类似物 新的磷酸蛋白及其类似物的活性研究 抑制磷酸钙盐的一次和二次沉淀。 最近，我们开发了一种新颖而有效的策略来准备任何 所需的他汀类和PRPS的合成含磷丝氨酸类似物 此外，还计划推出各种各样的此类类似产品。最近，它一直在 结果表明，当PRPS和他汀类药物吸附在磷灰石矿物上时， 可以促进几种突出的口腔细菌的黏附。 因此，我们也在计划化学合成类似物 施他林的C-末端和PRPS研究这些胶粘剂 分子水平上的反应。 我们正在继续研究这些抑制剂的演变，并正在 目前正在确定它们在红袋鼠唾液中的结构 和其他哺乳动物物种。

Human saliva is highly supersaturated with respect to basic calcium phosphate salts. However, precipitation of these salts from saliva and crystal growth of calcium phosphates onto dental enamel do not occur under normal physiological conditions. This unexpected stability is due to the inhibitory activities of two kinds of salivary phosphoproteins, statherin and the acidic proline-rich phosphoproteins (PRP). At physiological conditions, staterin inhibits spontaneous precipitation and crystal growth of calcium phosphates, whereas the PRP act as potent inhibitors of crystal growth by adsorbing onto surfaces of calcium phosphate minerals, such as dental enamel mineral. These activities are of biological significance in that they act to provide a supersaturated, but stable, protective and reparative environment for the dental enamel, which is important for the integrity of the teeth. The basic objective of this project is to relate the primary structure of these inhibitors to their functions in the oral cavity. The most rigorous way of attacking this problem is via chemical synthesis of analogs of these novel phosphoproteins and to study their activities of these analogs in inhibiting primary and secondary precipitation of calcium phosphate salts. Recently, we developed a novel and efficient strategy for preparing any desired synthetic phosphoserine-containing analog of staterin and the PRPs and a wide variety of such analogs are planned. Recently, it has been shown that the PRPs and statherin, when adsorbed onto apatitic minerals, can act to promote adhesion of several prominent oral bacteria. Consequently, we are also planning the chemical synthesis of analogs of the C-terminus of statherin and the PRPs to investigate these adhesive reactions on a molecular level. We are continuing to study the evolution of these inhibitors and are presently determining their structures in the saliva of the red kangaroo and other mammalian species.