IRON UTILIZATION BY BACTEROIDES GINGIVALIS
牙龈拟杆菌对铁的利用
基本信息
- 批准号:3222993
- 负责人:
- 金额:$ 11.1万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1991
- 资助国家:美国
- 起止时间:1991-11-01 至 1996-03-14
- 项目状态:已结题
- 来源:
- 关键词:Bacteroides gingivalis SDS polyacrylamide gel electrophoresis albumins bacterial proteins disease /disorder model electron microscopy gene mutation genetic library growth media haptoglobins heme hemoglobin hemopexin human tissue iron iron metabolism laboratory mouse lactoferrin microorganism culture microorganism growth molecular cloning mutant nutrient requirement nutrition related tag periodontitis siderophores succinates transferrin transposon /insertion element virulence western blottings
项目摘要
The anaerobic oral bacterium, Bacteroides gingivalis, has been implicated
as a major pathogen in adult periodontitis. Nutritional needs are of prime
ecological importance in the localization of B. gingivalis to the gingival
crevice area. B. gingivalis requires hemin for growth, however it is not
known if other iron-compounds can fulfill this nutritional requirement.
This study is designed to identify the mechanism of iron acquisition and
utilization, and to assess their role in pathogenicity of B. gingivalis.
The objectives of the proposed project are to define the iron requirements
of B. gingivalis for growth in vitro and to relate these to growth in vivo.
Iron sources will include iron bound to transferrin, lactoferrin, albumin,
haptoglobin and hemopexin, sources commonly found in vivo. The mechanisms
involved in iron acquisition will be examined by 1) determining if B.
gingivalis can utilize siderophores produced by other organisms, 2) if B.
gingivalis does indeed produce its own siderophores, and 3) if B.
gingivalis produces specific proteins that are regulated by growth in iron-
limited conditions. A genetic approach will be taken to examine more
closely the requirement for iron, by constructing strains of B. gingivalis
differing only in the ability to utilize hemin for growth. Tn4351
transpositional mutagenesis will be used to construct these strains.
Isogenic strains will be used in our mouse model to assess the virulence of
these strains. We will clone the corresponding genes for iron utilization
using specific Tn4351-B. gingivalis mutant genomic DNA as probes in total
genomic libraries. In addition, B. gingivalis grown in iron-sufficient and
iron-deficient medium will be evaluated for its ability to cause infection
in the mouse subcutaneous chamber model. These studies will allow for the
evaluation of the role of iron utilization in the pathogenicity of B.
gingivalis.
口腔厌氧细菌,牙龈拟杆菌,已牵连
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Caroline A Genco其他文献
Caroline A Genco的其他文献
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{{ truncateString('Caroline A Genco', 18)}}的其他基金
Porphyromonas gingivalis and Pancreatic Carcinogenesis in Mouse Models
小鼠模型中牙龈卟啉单胞菌与胰腺癌发生
- 批准号:
9519194 - 财政年份:2018
- 资助金额:
$ 11.1万 - 项目类别:
Microbial Disruption of Dendritic Cell Maturation and Function
树突状细胞成熟和功能的微生物破坏
- 批准号:
10237941 - 财政年份:2018
- 资助金额:
$ 11.1万 - 项目类别:
Microbial Disruption of Dendritic Cell Maturation and Function
树突状细胞成熟和功能的微生物破坏
- 批准号:
10468732 - 财政年份:2018
- 资助金额:
$ 11.1万 - 项目类别:
Microbial Disruption of Dendritic Cell Maturation and Function
树突状细胞成熟和功能的微生物破坏
- 批准号:
9790936 - 财政年份:2018
- 资助金额:
$ 11.1万 - 项目类别:
The Gonococcal Fur Regulon Link to Pathogenesis
淋球菌毛皮调节子与发病机制的联系
- 批准号:
9751634 - 财政年份:2017
- 资助金额:
$ 11.1万 - 项目类别:
Global Transcriptome Analysis of Mucosal Gonoccal Infection
粘膜淋菌感染的全局转录组分析
- 批准号:
9333190 - 财政年份:2016
- 资助金额:
$ 11.1万 - 项目类别:
TLR4 evasion, bacterial persistence and chronic inflammation
TLR4 逃避、细菌持续存在和慢性炎症
- 批准号:
8926492 - 财政年份:2014
- 资助金额:
$ 11.1万 - 项目类别:
TLR4 evasion, bacterial persistence and chronic inflammation
TLR4 逃避、细菌持续存在和慢性炎症
- 批准号:
9117800 - 财政年份:2014
- 资助金额:
$ 11.1万 - 项目类别:
Global transcriptome analysis of mucosal gonococcal infection
粘膜淋球菌感染的全局转录组分析
- 批准号:
9101453 - 财政年份:2014
- 资助金额:
$ 11.1万 - 项目类别:
Global transcriptome analysis of mucosal gonococcal infection
粘膜淋球菌感染的全局转录组分析
- 批准号:
8889364 - 财政年份:2014
- 资助金额:
$ 11.1万 - 项目类别: