IRON UTILIZATION BY BACTEROIDES GINGIVALIS

牙龈拟杆菌对铁的利用

基本信息

  • 批准号:
    3222993
  • 负责人:
  • 金额:
    $ 11.1万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1991
  • 资助国家:
    美国
  • 起止时间:
    1991-11-01 至 1996-03-14
  • 项目状态:
    已结题

项目摘要

The anaerobic oral bacterium, Bacteroides gingivalis, has been implicated as a major pathogen in adult periodontitis. Nutritional needs are of prime ecological importance in the localization of B. gingivalis to the gingival crevice area. B. gingivalis requires hemin for growth, however it is not known if other iron-compounds can fulfill this nutritional requirement. This study is designed to identify the mechanism of iron acquisition and utilization, and to assess their role in pathogenicity of B. gingivalis. The objectives of the proposed project are to define the iron requirements of B. gingivalis for growth in vitro and to relate these to growth in vivo. Iron sources will include iron bound to transferrin, lactoferrin, albumin, haptoglobin and hemopexin, sources commonly found in vivo. The mechanisms involved in iron acquisition will be examined by 1) determining if B. gingivalis can utilize siderophores produced by other organisms, 2) if B. gingivalis does indeed produce its own siderophores, and 3) if B. gingivalis produces specific proteins that are regulated by growth in iron- limited conditions. A genetic approach will be taken to examine more closely the requirement for iron, by constructing strains of B. gingivalis differing only in the ability to utilize hemin for growth. Tn4351 transpositional mutagenesis will be used to construct these strains. Isogenic strains will be used in our mouse model to assess the virulence of these strains. We will clone the corresponding genes for iron utilization using specific Tn4351-B. gingivalis mutant genomic DNA as probes in total genomic libraries. In addition, B. gingivalis grown in iron-sufficient and iron-deficient medium will be evaluated for its ability to cause infection in the mouse subcutaneous chamber model. These studies will allow for the evaluation of the role of iron utilization in the pathogenicity of B. gingivalis.
口腔厌氧细菌,牙龈拟杆菌,已牵连

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Caroline A Genco其他文献

Caroline A Genco的其他文献

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{{ truncateString('Caroline A Genco', 18)}}的其他基金

Porphyromonas gingivalis and Pancreatic Carcinogenesis in Mouse Models
小鼠模型中牙龈卟啉单胞菌与胰腺癌发生
  • 批准号:
    9519194
  • 财政年份:
    2018
  • 资助金额:
    $ 11.1万
  • 项目类别:
Microbial Disruption of Dendritic Cell Maturation and Function
树突状细胞成熟和功能的微生物破坏
  • 批准号:
    10237941
  • 财政年份:
    2018
  • 资助金额:
    $ 11.1万
  • 项目类别:
Microbial Disruption of Dendritic Cell Maturation and Function
树突状细胞成熟和功能的微生物破坏
  • 批准号:
    10468732
  • 财政年份:
    2018
  • 资助金额:
    $ 11.1万
  • 项目类别:
Microbial Disruption of Dendritic Cell Maturation and Function
树突状细胞成熟和功能的微生物破坏
  • 批准号:
    9790936
  • 财政年份:
    2018
  • 资助金额:
    $ 11.1万
  • 项目类别:
The Gonococcal Fur Regulon Link to Pathogenesis
淋球菌毛皮调节子与发病机制的联系
  • 批准号:
    9751634
  • 财政年份:
    2017
  • 资助金额:
    $ 11.1万
  • 项目类别:
Global Transcriptome Analysis of Mucosal Gonoccal Infection
粘膜淋菌感染的全局转录组分析
  • 批准号:
    9333190
  • 财政年份:
    2016
  • 资助金额:
    $ 11.1万
  • 项目类别:
TLR4 evasion, bacterial persistence and chronic inflammation
TLR4 逃避、细菌持续存在和慢性炎症
  • 批准号:
    8926492
  • 财政年份:
    2014
  • 资助金额:
    $ 11.1万
  • 项目类别:
TLR4 evasion, bacterial persistence and chronic inflammation
TLR4 逃避、细菌持续存在和慢性炎症
  • 批准号:
    9117800
  • 财政年份:
    2014
  • 资助金额:
    $ 11.1万
  • 项目类别:
Global transcriptome analysis of mucosal gonococcal infection
粘膜淋球菌感染的全局转录组分析
  • 批准号:
    9101453
  • 财政年份:
    2014
  • 资助金额:
    $ 11.1万
  • 项目类别:
Global transcriptome analysis of mucosal gonococcal infection
粘膜淋球菌感染的全局转录组分析
  • 批准号:
    8889364
  • 财政年份:
    2014
  • 资助金额:
    $ 11.1万
  • 项目类别:
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