IMMUNOGENICITY OF FIMBRILLIN FROM B. GINGIVALIS

来自牙龈芽孢杆菌的纤丝蛋白的免疫原性

• 批准号: 3223210
• 负责人: Patrick Michael Flood
• 金额: $ 16.56万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-04-01 至 1996-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3223210
• 关键词: Bacteroides gingivalis; T cell receptor; T lymphocyte; antigen presenting cell; bacterial antigens; bacterial proteins; cellular immunity; enzyme linked immunosorbent assay; genes; helper T lymphocyte; humoral immunity; hybridomas; immunogenetics; in situ hybridization; interferons; interleukin 2; interleukin 4; laboratory mouse; lymphokines; major histocompatibility complex; messenger RNA; northern blottings; peptides; periodontium disorder; pilus; protein purification; protein sequence; synthetic peptide; virulence; western blottings

The specific immune response to bacterial infections have been shown to involve the generation of both cellular and humoral immunity directed against bacterial antigens. The activation of these immunologic effector mechanisms is accomplished by the interaction of inducer T cells with immunologic effector cells, either B cells, T cells, or macrophages. Severe adult Periodontal disease is a disorder characterized by the inability to resolve the infection by a pathogen or pathogens within the gingival margins and the periodontium which results in a chronic destructive immunologic response. Bacteroides Gingivalis, a black pigmented gram negative bacteria, has been implicated as one of the major causative agents in severe adult periodontal disease. This proposal describes studies that investigate the nature and specificity of T lymphocyte response to fimbrillin, the constitutive protein of fimbriae of B. Gingivalis strain 381. T cells recognize antigen on antigen-presenting cells as peptides associated with self-molecules encoded by the Major Histocompatibility Complex. Therefore, the immunogenicity of whole Bg, bacterial fimbrae, or purified fimbrillin molecules will be analyzed, and factors influencing the immunogenicity of these molecules compared. Small -synthetic peptides of the fimbrillin molecule from B. Gingivalis strain 381 will be generated based on the predicted amino acid sequence from the cloned fimbrillin gene, and these peptides will be analyzed in order to identify specific amino acid sequences, i.e. epitopes, on the fimbrillin molecule which induce immunologic responses in T lymphocytes. The nature of the T cell response to immunologic epitopes will be analyzed for its specificity for MHC-linked molecules, its production of inflammatory and humoral lymphokines, and its ability to respond to Bg, fimbrae, or fimbrillin antigen on a variety of different antigen presenting cells. Understanding the factors which influence the immune response to immunogenic epitopes on fimbrillin will significantly enhance our understanding of how T cells respond to bacterial antigens in .vivo, and will aid in designing better immunologic -approaches to the treatment of periodontal disease.

对细菌感染的特异性免疫反应已被证明是 涉及细胞和体液免疫的产生， 对抗细菌抗原。 这些免疫效应物的激活 机制是通过诱导T细胞与 免疫效应细胞，B细胞、T细胞或巨噬细胞。 严重的成人牙周病是一种疾病，其特征在于， 无法解决病原体或病原体内的感染 牙龈边缘和牙周组织，导致慢性 破坏性免疫反应 牙龈拟杆菌，黑色 色素革兰氏阴性菌，已被牵连作为一个主要的 严重成人牙周病的病因。 这项建议 描述了研究T细胞的性质和特异性的研究， 淋巴细胞对菌毛蛋白的反应，菌毛的组成蛋白， B。 第381章. T细胞将抗原呈递细胞上的抗原识别为肽 与主要组织相容性编码的自身分子相关 复杂. 因此，全Bg、细菌菌毛或 将分析纯化的菌毛蛋白分子，并分析影响菌毛蛋白分子的因素。 比较这些分子的免疫原性。 小分子合成肽 来自B的菌毛蛋白分子。将产生牙龈菌菌株381 基于来自克隆的菌毛蛋白基因的预测氨基酸序列， 这些肽将被分析，以确定特定的氨基酸， 在菌毛蛋白分子上的酸性序列，即表位， T淋巴细胞的免疫应答。 T细胞反应的本质 免疫表位将分析其对MHC相关抗原的特异性。 分子，其炎症和体液淋巴因子的产生，及其 在多种免疫系统上对Bg、菌毛或菌毛蛋白抗原应答的能力 不同的抗原呈递细胞 了解哪些因素 影响对菌毛蛋白上免疫原性表位的免疫应答将 这大大提高了我们对T细胞如何对细菌应答的理解， 抗原，并将有助于设计更好的免疫方法 涉及牙周病的治疗。