ACID-BASE BALANCE REGULATION OF RENAL METABOLISM

肾脏代谢的酸碱平衡调节

• 批准号: 3226000
• 负责人: DAVID P SIMPSON
• 金额: $ 18.56万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-12-01 至 1990-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3226000
• 关键词: acid base balance; acidosis; alkalosis; ammonia; anions; bicarbonates; citrates; dogs; glutamine; high performance liquid chromatography; homeostasis; ion transport; kidney metabolism; laboratory rabbit; laboratory rat; membrane permeability; membrane potentials; mitochondrial membrane; oxoglutarate dehydrogenase; phosphates; radiotracer; renal tubular transport; renal tubule; respiratory acidosis; tissue /cell culture; ultracentrifugation

This project is an investigation into regulation of intermediary metabolism in the kidney by acid-base changes and the relationship to physicologic processes involved in acid-base homeostasis. The emphasis in the project is to relate properties of the inner mitochondrial membrane to two different metabolic responses by the kidney to alterations in acid-base balance. (1) During chronic metabolic acidosis renal ammoniagenesis from glutamine increases strikingly. Previous work has suggested that a major factor in this adaptive response is an increase in acidosis in glutamine transport by its carrier in the inner mitochondrial membrane, leading to delivery of more glutamine to phosphate dependent glutaminase. Further evidence for adaptation of the glutamine transporter will be sought in studies on mitochondria from dogs with chronic metabolic or respiratory acidosis. Changes in mitochondrial glutamine transport and metabolism will be correlated with NH4+excretion, NH3 synthesis and glutamine utilization in intact animals with chronic metabolic or respiratory acidosis. (2) Levels in renal cortex of a number of citric acid cycle and related substrates decrease in acute metabolic acidosis and increase in metabolic alkalosis. We postulate that these changes are the result of substrate shifts occurring in response to variations in the mitochondrial pH gradient. Studies will be carried out in isolated kidney tubules and in mitochondria to determine the validity of this hypothesis.

本项目是对中间代谢调控的研究

项目成果

Substrate responses to acid-base alterations in dog renal proximal tubules.

狗肾近曲小管酸碱变化的底物反应。

• DOI: 10.1152/ajprenal.1992.262.6.f1039
• 发表时间: 1992
• 期刊: The American journal of physiology
• 作者: Simpson,DP;McSherry,N;Scarbrough,E;Zweifel,K
• 通讯作者: Zweifel,K

Hyperplasia precedes increased glomerular filtration rate in rat remnant kidney.

在大鼠残肾中，增生先于肾小球滤过率增加。

• DOI: 10.1038/ki.1990.43
• 发表时间: 1990
• 期刊: Kidney international
• 影响因子: 19.6
• 作者: Miskell,CA;Simpson,DP
• 通讯作者: Simpson,DP