STRUCTURE AND FUNCTION OF PHOSPHOFRUCTOKINASE

我们的长期目标是了解 各种哺乳动物组织和酵母中的碳水化合物代谢。 具体而言，我们感兴趣的是 磷酸果糖激酶，糖酵解的关键酶之一。 目前，我们正在研究的生理重要性， 肝和心脏中磷酸果糖激酶的共价修饰。 我们也在研究果糖-2，6-P2的作用，最近， 发现了磷酸果糖激酶的有效激活剂。 合成 和果糖-2，6-P2的降解是由双功能催化剂催化的， 酶，果糖-6-P，2-激酶和果糖-2，6-二磷酸酶。 我们计划纯化和表征心脏果糖-6-P，2- 激酶：果糖-2，6-二磷酸酶。 结构研究 肝脏双功能酶的功能将 持续期间内的 对这种双功能酶特别感兴趣的是 磷酸化导致相反的活动，这取决于 组织来源。 使用灌注心脏和分离的心肌细胞 磷酸化的生理意义将是 以及各种α和β的影响 激动剂和心脏工作的双功能酶， 将研究磷酸果糖激酶。

Our long term goal is to understand the control mechanisms of carbohydrate metabolism in various mammalian tissues and yeast. Specifically, we are interested in the regulation of phosphofructokinase, one of the key enzymes of glycolysis. Currently we are investigating the physiological importance of a covalent modification of phosphofructokinase in liver and heart. We are also studying the roles of fructose-2, 6-P2, a recently discovered potent activator of phosphofructokinase. Synthesis and degradation of fructose-2,6-P2 is catalyzed by a bifunctional enzyme, fructose-6-P,2-kinase and fructose-2,6-bisphosphatase. We plan to purify and characterize heart fructose-6-P, 2- kinase:fructose-2, 6-bisphosphatase. The study of the structure and the function of the liver bifunctional enzyme will be continued. Of particular interest with this bifunctional enzyme is phosphorylation which leads to opposite activities depending on the tissue sources. Using perfused heart and isolated myocytes the physiological significance of phosphorylation will be investigated as well as the effects of various alpha and beta agonists and cardiac work on the bifunctional enzymes and also on phosphofructokinase will be studied.