INTESTINAL CELL MEMBRANES: STRUCTURE AND FUNCTION
肠细胞膜:结构和功能
基本信息
- 批准号:3227579
- 负责人:
- 金额:$ 14.17万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1979
- 资助国家:美国
- 起止时间:1979-08-01 至 1993-01-31
- 项目状态:已结题
- 来源:
- 关键词:Golgi apparatus affinity chromatography basement membrane basolateral membrane cell adhesion cell differentiation cell membrane forskolin gastrointestinal absorption /transport glycoproteins high performance liquid chromatography intestines laboratory rabbit laboratory rat membrane structure oligosaccharides secretion tissue /cell culture
项目摘要
The long-term objective is to define the role of enterocyte
plasmelemmal glyco-proteins in the control of its differentiation
into a functional absorptive cell. Work over the last 25-years
have indicated that the oligosaccharides of plasmalemmal
glycoproteins influence cell differentiation as cell-cell
recognition and as cell adhesion factors. The latter are important
in determining tissue organization during differentiation
particularly where the separation of the plasmalemma into domains
may determine vector-directed cell function as in absorption. Our
specific aims are; 1) to determine the structural changes in
enterocyte plasmalemmal oligosaccharides associated with enterocyte
differentiation and cell polarity, and 2) to relate these changes
to enterocyte-to-intestinal basement membrane (IBM) adhesion, and
interrelationship critical to tissue organization and normal
intestinal absorption. Rat enterocytes will be isolated in
fractions representative of the crypt-to-villus gradient of
differentiation. The different domains (i.e., microvillus,
lateral, and basal) of the enterocyte plasmalemma will be separated
as well as the different parts of the Golgi. Nucleotide-sugar
translocation and newly defined enzymes specific for different
Golgi subunits will be used. A basal-enriched membrane will be
detected by antibodies to glycoproteins immunocytologically
associated with the basal plasmalemma. The possibility that IBM
components retain association with basal membrane or that the basal
domain has specific IBM receptors will be tested. Oligosaccharides
isolated from these plasmalemmal domains will be analyzed by
controlled hydrolysis, substrate and lectin affinity chromatography
and by GLC and HPLC separation. Initially the goal will be to
determine the oligosaccharide structures most characteristic of a
particular plasmalemmal domain. The relationship of these
oligosaccharides to cell-IBM adhesion will be studied using assays
for cell adhesion; matrices derived from IBM will be used to test
adhesion of tissue culture cells and of isolated enterocytes.
these studies should provide information on how enterocyte polarity
is developed and maintained during differentiation. Incomplete
differentiation and decreased enterocyte polarity are general
expressions of enterocyte injury and malfunction seen in many
intestinal diseases. Our research should contribute to an
understanding of the fundamental cell biology controls in tissue
organization so important to intestinal absorption and secretion.
长期目标是确定肠上皮细胞的作用,
质膜糖蛋白在其分化的控制
转化为功能性吸收细胞 过去25年的工作
已经表明质膜的寡糖
糖蛋白影响细胞间分化
识别和作为细胞粘附因子。 后者很重要
在分化过程中决定组织结构
特别是当质膜分离成结构域时
可以决定载体导向的细胞功能,如吸收。 我们
具体目标是:1)确定结构变化,
肠细胞质膜低聚糖
分化和细胞极性,以及2)将这些变化
肠细胞-肠基底膜(IBM)粘附,和
对组织组织和正常
肠吸收 大鼠肠细胞将在
代表隐窝至绒毛梯度的分数
分化 不同的域(即,微绒毛,
外侧和基底)的肠上皮细胞质膜将被分离
以及高尔基体的不同部分。 核苷酸糖
易位和新定义的酶特异性不同
将使用高尔基体亚基。 基底富集膜将被
通过免疫细胞学的糖蛋白抗体检测
与基底质膜相连。 IBM可能
成分与基底膜保持联系,或者基底膜
域具有特定的IBM受体将被测试。 寡糖
从这些质膜结构域分离的蛋白质将通过
控制水解、底物和凝集素亲和层析
并通过GLC和HPLC分离。 最初的目标是
确定最具特征的寡糖结构,
特别是质膜结构域。 这些关系
将使用测定来研究寡糖与细胞-IBM粘附
用于细胞粘附;来自IBM的基质将用于测试
组织培养细胞和分离的肠上皮细胞的粘附。
这些研究应该提供肠上皮细胞极性
是在分化过程中形成和维持的。 不完全
分化和肠上皮细胞极性降低是普遍的
肠上皮细胞损伤和功能障碍的表达在许多
肠道疾病。 我们的研究应该有助于
了解组织中的基本细胞生物学控制
组织对肠道吸收和分泌非常重要。
项目成果
期刊论文数量(0)
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科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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MILTON M WEISER其他文献
MILTON M WEISER的其他文献
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{{ truncateString('MILTON M WEISER', 18)}}的其他基金
INTESTINAL CA2+ TRANSPORT: VITAMIN D-DEPENDENT MECHANISM
肠道 CA2 转运:维生素 D 依赖性机制
- 批准号:
3230778 - 财政年份:1983
- 资助金额:
$ 14.17万 - 项目类别:
INTESTINAL CA2 + TRANSPORT: VIT. D-DEPENDENT MECHANISMS
肠道 CA2 运输:VIT。
- 批准号:
3152494 - 财政年份:1983
- 资助金额:
$ 14.17万 - 项目类别:
INTESTINAL CA2+ TRANSPORT: VITAMIN D-DEPENDENT MECHANISM
肠道 CA2 转运:维生素 D 依赖性机制
- 批准号:
3230776 - 财政年份:1983
- 资助金额:
$ 14.17万 - 项目类别:
INTESTINAL CA2+ TRANSPORT: VITAMIN D-DEPENDENT MECHANISM
肠道 CA2 转运:维生素 D 依赖性机制
- 批准号:
3230779 - 财政年份:1983
- 资助金额:
$ 14.17万 - 项目类别:
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