MUSCARINIC RECEPTOR REGULATION OF THE ANTERIOR PITUITARY

垂体前叶的毒蕈碱受体调节

• 批准号: 3230433
• 负责人: GARY D SNYDER
• 金额: $ 8.25万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-01-01 至 1987-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3230433
• 关键词: acetylcholine; cell type; dopamine; embryo /fetus tissue /cell culture; hormone inhibitor; hormone regulation /control mechanism; hypothalamic pituitary axis; hypothalamus; neural information processing; neuroendocrine system; prolactin releasing /inhibiting factor; radioimmunoassay; scintillation counter; secretion; somatostatin; somatotropin; thyrotropin releasing hormone; vascular smooth muscle nervous control; vasoactive intestinal peptide

Muscarinic cholinergic receptors have been found to exist on the membranes of anterior pituitary cells. Although the receptors seem to regulate secretion of both prolactin (PRL) and growth hormone (GH), no good evidence is available as to which of the several cell types present in the anterior pituitary has the receptors. Furthermore, there are no data describing the physiological significance of these receptors. This proposal will characterize the muscarinic receptor in the rat anterior pituitary as to: 1) number and location e.g., cell type, 2) physiological significance in controlling GH and PRL secretion; 3) the interplay of various hypothalamic releasing/inhibiting factors. The project will make use of several major types of methodology: The muscarinic receptor population will be studied both on intact cells maintained in culture and on membranes isolated from animals in various physiological states. Use of cell separation technology and electron microscopy will aid in localization of the muscarinic receptors to specific cell types and thus support the localization obtained physiologically via hormone secretion experiments. The effect of interaction of peptides (such as somatostatin, vasoactive intestinal peptide and thyroid stimulating hormone-releasing hormone) found in the hypothalamus and muscarinic receptor agonists (e.g., acetylcholine and oxotremorine) on secretion of hormones and binding to the receptor will be described, then correlated, in an effort to define the physiological relevance of the muscarinic receptor to regulation of GH and PRL secretion. The results of these studies may provide new possibilities for pharmacological control of anterior pituitary hormones, especially GH and PRL.

毒蕈碱胆碱能受体已被发现存在于细胞膜上 脑垂体前叶细胞 虽然受体似乎调节 泌乳素（PRL）和生长激素（GH）分泌，无良好证据 是可用的，以目前在前几个细胞类型 垂体有受体。 此外，没有数据说明 这些受体的生理意义。 这项建议会 将大鼠垂体前叶中的毒蕈碱受体表征为： 1)号码和位置，例如，细胞类型，2）生理意义， 控制GH和PRL分泌; 3）各种下丘脑的相互作用 释放/抑制因子。 该项目将利用几个主要 方法学类型：将研究毒蕈碱受体群体 在保持在培养物中的完整细胞上和在分离自 各种生理状态下的动物。 使用细胞分离技术 电子显微镜可以帮助定位毒蕈碱 受体的特定细胞类型，从而支持获得的定位 通过激素分泌实验进行生理学研究。 的影响 肽（如生长抑素、血管活性肠肽） 肽和促甲状腺激素释放激素）， 下丘脑和毒蕈碱受体激动剂（例如，乙酰胆碱和 oxotremorine）对激素分泌和受体结合的影响将是 描述，然后关联，以定义生理 毒蕈碱受体与GH和PRL调节的相关性 分泌物 这些研究的结果可能为 垂体前叶激素的药理学控制，尤其是GH， 催乳素