Tissue Culture Hybridoma Core

组织培养杂交瘤核心

• 批准号: 7900766
• 负责人: GARY D SNYDER
• 金额: $ 8.73万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7900766
• 关键词: Antibiotics; Antibodies; Back; Biology; Biomedical Research; Cancer Center Support Grant; Cell Culture Techniques; Cell Line; Charge; Culture Media; Funding; Future; Growth Factor; Hybridomas; Marketing; Molecular Biology Techniques; Monoclonal Antibodies; Mycoplasma; Operative Surgical Procedures; Price; Production; Reagent; Research Personnel; Sales; Scientist; Screening procedure; Serum; Services; Soil; Source; Surveys; Testing; Transfection; United States National Institutes of Health; anticancer research; cost; cost efficient; discount; hybridoma production; member; monoclonal antibody production; restriction enzyme; tissue culture; tool

Tissue Culture Hybridoma Core (TCHC) Tissue Culture and Monoclonal Antibodies are central tools in cancer research. The Tissue Culture/Hybridoma Core (TCHC) provides a variety of services to HCCC members in a cost efficient setting including 1) Production of hybridoma cell lines 2) Production, quantification, and isotyping of antibodies 3) Mycoplasma screening. The TCHC also stores clones for future use by the investigator 4) Providing a ready and low cost source of a wide variety of discounted tissue culture media (15 common media) and other reagents including various sera, media supplements, growth factors, transfection reagents and antibiotics 5) A "store-front" operation that provides restriction enzymes and a broad variety of biomedical research products to investigators in small quantities at < commercial prices This facility is used by more than 80% of HCCC researchers, and surveys confirm it is highly valued by HCCC members.

组织培养杂交种核心（TCHC） 组织培养和单克隆抗体是癌症研究的核心工具。组织 Culture/Hybridization Core（TCHC）以具有成本效益的方式为HCCC成员提供各种服务 包括 1)杂交瘤细胞系的产生 2)抗体的产生、定量和同种型 3)支原体筛查。TCHC还存储克隆供研究者将来使用 4)提供各种折扣组织培养基（15种常用培养基）和其他试剂（包括各种血清、培养基补充剂、生长因子、转染试剂和抗生素）的现成和低成本来源 5)以<商业价格向研究人员提供少量限制性内切酶和各种生物医学研究产品的“店面”业务 该设施被超过80%的HCCC研究人员使用，调查证实它受到HCCC成员的高度重视。