HUMAN MONOCLONAL ANTIBODIES

我们的目标是确定自身免疫反应的性质， 慢性特发性血小板减少性紫癜（ITP）患者。 期间 在过去的一年里，我们已经分离出一系列杂交细胞系， 抗血小板抗原的人自身抗体。 这些混血儿 通过将来自ITP患者的EBV转化的淋巴细胞与 抗6-TG NS-1小鼠骨髓瘤-人B淋巴细胞异源杂交细胞 行，F6。 两种杂交细胞系分泌的单克隆抗体 已被鉴定为与血小板蛋白或糖蛋白反应， 分子量约为10万道尔顿， 由另一条线分泌的反应与一系列 血小板相关蛋白或糖蛋白。 具体来说，我们现在希望1）制备体细胞杂交体文库 其分泌人抗血小板自身抗体;并检查2） 血清学和生化特异性，以及3）同种型和 单克隆抗血小板自身抗体的独特型特征。 然后，我们将使用这些试剂来确定 血清抗血小板同种型、独特型及分子特异性 抗体和疾病结果。 我们的长远目标是利用这个 信息开发免疫方法治疗慢性 ITP。

Our objective is to define the nature of the autoimmune response in patients with chronic idiopathic thrombocytopenic purpura (ITP). During the past year we have isolated a series of hybrid cell lines that produce human autoantibodies against platelet antigens. These hybrids were prepared by fusing EBV transformed lymphocytes from patients with ITP with a 6TG resistant NS-1 mouse myeloma-human B lymphocyte heterohybrid cell line, F6. Monoclonal antibody secreted by two of the hybrid cell lines have been identified to react with a platelet protein or glycoprotein with a molecular weight of approximately 100,000 daltons, and an antibody secreted by another line reacts with a determinant shared by a series of platelet associated proteins or glycoproteins. Specifically, we now wish to 1) prepare a library of somatic cell hybrids which secrete human antiplatelet autoantibody; and examine 2) the serological and biochemical specificity, as well as 3) the isotypic and idiotypic characteristics of the monoclonal antiplatelet autoantibodies. We will then use these reagents to 4) determine the relationship between the isotype, idiotype, and molecular specificity of serum antiplatelet antibodies and disease outcome. Our long-range goal is to use this information to develop immunologic methods for the treatments of chronic ITP.