BIOCHEMISTRY OF HUMAN ALPHA-GALACTOSIDASE A

人类 α-半乳糖苷酶 A 的生物化学

• 批准号: 3235587
• 负责人: VINCENT J. KIDD
• 金额: $ 9.37万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-06-01 至 1989-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3235587
• 关键词: Fabry's disease; alpha galactosidase; biological polymorphism; enzyme structure; gene expression; genetic disorder diagnosis; genetic library; genetic manipulation; genetic mapping; genetic recombination; human tissue; immunochemistry; liver metabolism; molecular cloning; molecular genetics; molecular pathology; mutant; nucleic acid sequence; prenatal diagnosis; proteolysis

Alpha-galactosidase A is a lysosomal enzyme that catalyzes the conversion of ceramide trihexoside to lactosylceramide and galactose. This enzyme activity is found in many tissues of normal individuals but its absence leads to a metabolic block in the lysosomal degradation of glycoshpingolipids. The physiological role of alpha-galactosidase A in lysosomal degradation of certain proteins is further defined by the fact that individuals with Fabry's disease lack alpha-galactosidase A activity in their visceral tissues. Due to the fact that alpha-galactosidase A is found in very low quantities in normal human tissues and it is fairly labile, its biochemical and molecular properties are not well defined. For example, the alpha-galactosidase A protein has a molecular weight range ot 49,000 - 101,000 and no accurate amino acid sequence data is available. Alpha-galactosidase A is synthesized in many tissues in vivo, but it is found to represent approximately 0.02% of the total liver proteins. Using an affinity purified rabbit polyclonal antibody to human alpha-galactosidase A, we have demonstrated its monospecificity and screened a human liver cDNA library constructed using the expression vector gt-11. A putative human alpha-galactosidase A clone was identified after screening 2 million recombinants in the library. The authenticity of the cDNA clone was verified by demonstrating the corresponding human chromosomal gene maps to the x chromosome, which is the known locus for Fabry's disease in man. This was accomplished by using the putative alpha--galactosidase A cDNA clone to probe both gene dosage and somatic cell hybrid DNA panels. Having isolated a human alpha-galactosidase A cDNA clone, we propose to perform detailed biochemical and molecular characterization of human alpha-galactosidase A. A full-length cDNA clone will be isolated and its nucleotide sequence determined, from which the amino acid sequence of the protein will be deduced. The chromosomal alpha-galactosidase A gene will be isolated and its molecular structure will be defined. Mutant alpha-galactosidase A genes will also be isolated from Fabry's patients and sequenced. These studies will define the molecular basis of Fabry's disease, and should provide the foundation for development of analytical methodologies for prenatal diagnosis and carrier detection of the herediatry disorder by gene mapping.

α-半乳糖苷酶A是一种溶酶体酶， 将神经酰胺三己糖苷转化为乳糖神经酰胺和半乳糖。 这种酶 在正常人的许多组织中发现了活性， 导致溶酶体降解的代谢阻断， 糖脂。 α-半乳糖苷酶A的生理作用 某些蛋白质的溶酶体降解被进一步定义为 法布里病患者缺乏α-半乳糖苷酶A活性 在内脏组织中。 由于α-半乳糖苷酶A是 在正常人体组织中含量很低， 不稳定，其生物化学和分子特性尚未明确。 为 例如，α-半乳糖苷酶A蛋白的分子量范围为 49，000 - 101，000，没有准确的氨基酸序列数据。 α-半乳糖苷酶A在体内的许多组织中合成，但它是 发现约占总肝蛋白的0.02%。 使用 亲和纯化的兔抗人多克隆抗体 α-半乳糖苷酶A，我们已经证明了它的单特异性， 筛选用该表达载体构建的人肝cDNA文库 gt-11。 鉴定了一个假定的人α-半乳糖苷酶A克隆， 在文库中筛选200万个重组体。 的真实性 cDNA克隆通过证明相应的人 染色体基因映射到x染色体，这是已知的基因座， 这是通过使用假定的 α-半乳糖苷酶一个cDNA克隆，用于探测基因剂量和体细胞 细胞杂交DNA板。 分离了人α-半乳糖苷酶A cDNA， 克隆，我们建议进行详细的生化和分子 人α-半乳糖苷酶A的表征。 全长cDNA克隆 将被分离并确定其核苷酸序列， 推导出该蛋白的氨基酸序列。 染色体 α-半乳糖苷酶A基因将被分离，其分子结构 将被定义。 突变的α-半乳糖苷酶A基因也将被分离出来 并进行了测序 这些研究将确定 法布里病的分子基础，并应提供基础， 发展产前诊断和携带者的分析方法 通过基因定位检测遗传性疾病。