ELECTROPHYSIOLOGY OF GASTRIC ION TRANSPORT

胃离子传输的电生理学

• 批准号: 3238092
• 负责人: JEFFREY DEMAREST
• 金额: $ 9.43万
• 依托单位: UNIVERSITY OF ARKANSAS AT FAYETTEVILLE
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-01 至 1993-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3238092
• 关键词: Urodela; antiport; carbonates; electrophysiology; epithelium; gastrointestinal absorption /transport; ion transport; membrane permeability; membrane potentials; microelectrodes; mucosa; sodium potassium exchanging ATPase

The overall goals of this project are to use electrophysiological methods to: (i) determine the membrane mechanisms which oxyntic (OC, acid-secreting) and surface epithelial (SEC, mucus- secreting) cells of the gastric fundus use to actively transport H, Cl, Na and HC03: and (ii) characterize the properties of the paracellular shunt. Because SECs of fundus and the cells of antrum have very similar transport properties, measurements will also be extended to the antrum. The isolated Necturus gastric mucosa and antrum will be used because they exhibit the same ionic transport properties as their mammalian counterparts yet they are histologically simpler, have larger cells and are much more robust in vitro. A vibrating probe which measures small extracellular currents will be used to determine the conductance properties of the SECs and OCs individually. Standard microelectrodes are used to impale both SECs and OCs and obtain membrane potential measurements. The vibrating probe and microelectrodes will be used in combination to obtain quantitative measurements of the conductance properties of the mucosal and serosal membranes and shunts (between cells) of the two cell types. Preliminary data indicate that SECs and OCs have very different membrane transport and conductance properties and also different shunts. Double-barreled ion selective electrodes will be used to determine intracellular (K), (Cl), (Na) and pH in the steady state and also to assess the non-conductive movements of ions via cotransporters (e.g. KCl symport) and exchangers (e.g. Cl/HC03 or Na/H antiport). Using these techniques the permeability, conductive and transport properties of the mucosal and serosal membranes of OCs and SECs and SECs to Na+, K+, Cl- HC03 and H+ will be determined. Also, the conductance properties of the shunts between OCs and between SECS can be determined. This information will be of obvious importance for our overall knowledge of how the stomach works. Also, since the "barrier" function of the stomach must somehow be related to the ability of the cells and shunt to exclude H+, this work should also yield useful information about this question.

这个项目的总体目标是利用电生理

项目成果

Electrophysiology of the parietal cell.

壁细胞的电生理学。

• DOI: 10.1146/annurev.ph.52.030190.001515
• 发表时间: 1990
• 期刊: Annual review of physiology
• 影响因子: 18.2
• 作者: Demarest,JR;Loo,DD
• 通讯作者: Loo,DD

H and HCO3 transport across the basolateral membrane of the parietal cell.

H 和 HCO3 穿过壁细胞的基底外侧膜运输。

• DOI: 10.1111/j.1749-6632.1989.tb25183.x
• 发表时间: 1989
• 期刊: Annals of the New York Academy of Sciences
• 影响因子: 5.2
• 作者: Machen,TE;Townsley,MC;Paradiso,AM;Wenzl,E;Negulescu,PA
• 通讯作者: Negulescu,PA

pHi dependence of Na-H exchange and H delivery in IEC-6 cells.

IEC-6 细胞中 Na-H 交换和 H 输送的 pHi 依赖性。

• DOI: 10.1152/ajpcell.1992.262.1.c164
• 发表时间: 1992
• 期刊: The American journal of physiology
• 作者: Sjaastad,MD;Wenzl,E;Machen,TE
• 通讯作者: Machen,TE

Intracellular Ca2+ signalling is modulated by K+ channel blockers in colonic epithelial cells (HT-29/B6).

结肠上皮细胞 (HT-29/B6) 中的 K 通道阻滞剂可调节细胞内 Ca2 信号传导。

• DOI: 10.1007/bf00381512
• 发表时间: 1992
• 期刊: Pflugers Archiv : European journal of physiology
• 作者: Illek,B;Fischer,H;Machen,TE
• 通讯作者: Machen,TE

Activation of apical chloride channels in the gastric oxyntic cell.

胃泌酸细胞中顶端氯离子通道的激活。

• DOI: 10.1126/science.2474200
• 发表时间: 1989
• 期刊: Science (New York, N.Y.)
• 作者: Demarest,JR;Loo,DD;Sachs,G
• 通讯作者: Sachs,G