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Quantification of the mechanisms of light tolerance that determine growth and productivity in plants and algae.

确定植物和藻类生长和生产力的耐光机制的量化。

基本信息

批准号：
BB/R015694/1
负责人：
Alexander Ruban
金额：
$ 49.28万
依托单位：
Queen Mary University of London
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2018
资助国家：
英国
起止时间：
2018 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FR015694%2F1
关键词：
Quantification mechanisms light tolerance determine

项目摘要

This proposal addresses the rapid adaptation of plants and algae to frequent changes in the light environment: non-photochemical quenching (NPQ). The molecular basis of NPQ and the assessment of NPQ effectiveness and high light tolerance in photosynthetic organisms are the focus of this proposal. The mechanism behind the adaptive reorganisation of the photosynthetic apparatus in response to high light intensities will be reproduced in membranes selectively devoid of its components and studied using electron microscopy, spectroscopy and biochemistry approaches. The role of specific proteins PsbS and Lhcx in the process will be addressed. A recently-developed novel method will be applied to determine effectiveness of NPQ and high light tolerance of plants and algae - a so-called pNPQ technology. This knowledge is important for understanding the patterns of the evolution of photosynthetic organisms in changing environments, allowing for the prediction of the impact of the climate change upon our planet's plant and algal communities. The proposal is timely since the PI has discovered the PsbS protein interaction patterns with the components of the photosynthetic apparatus, the economic nature of NPQ and visualised specific clustering of the light-collecting proteins (LHCII) in the photosynthetic membrane. Further, the recent paper of Niyogi's and Long's laboratories (https://www.ncbi.nlm.nih.gov/pubmed/27856901) benefited from the use of pNPQ-based technology that helped to develop better performing crop plants just by making their NPQ respond more quickly to light fluctuations. In addition, the company Optisciences (USA) has utilised the pNPQ methodology in their revolutionary plant monitoring fluorimeter PSP32 (http://www.optisci.com/psp32.html) that is indispensable for monitoring plant light tolerance in the field. Hence, the further impact of the current proposal on research dealing with crop improvement via enhancement of energy-harvesting or light-endurance properties is expected.

这项建议解决了植物和藻类对光环境中频繁变化的快速适应：非光化学猝灭(NPQ)。NPQ的分子基础以及NPQ的有效性和光合作用生物体的高光耐受性的评估是这一提议的重点。光合作用装置对强光的适应性重组背后的机制将在选择性地不含其成分的膜上复制，并使用电子显微镜、光谱学和生物化学方法进行研究。将讨论特定蛋白质PSB和Lhcx在这一过程中的作用。最近开发的一种新方法将被应用于测定NPQ的有效性以及植物和藻类的高光耐受性--所谓的pNPQ技术。这一知识对于理解光合作用有机体在不断变化的环境中的演化模式非常重要，从而能够预测气候变化对地球上植物和藻类群落的影响。这项建议是及时的，因为PI已经发现了PSB蛋白质与光合作用装置组件的相互作用模式、NPQ的经济性质以及光合膜中光收集蛋白质(LHCII)的可视化特定聚集。此外，Niyoi和Long的实验室(https://www.ncbi.nlm.nih.gov/pubmed/27856901)最近的论文受益于基于pnpq的技术的使用，这种技术通过使它们的npq对光波动做出更快的反应来帮助培育出表现更好的作物。此外，OptiSciences(美国)公司在其革命性的植物监测荧光仪PSP32(http://www.optisci.com/psp32.html))中使用了pNPQ方法，这是在野外监测植物耐光性所不可或缺的。因此，目前的提议对通过增强能量收集或耐光性能来改进作物的研究将产生进一步的影响。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

The Mechanism of Nonphotochemical Quenching: The End of the Ongoing Debate.

非光化学猝灭机制：持续争论的结束。

DOI：
10.1104/pp.19.00538
发表时间：
2019
期刊：
Plant physiology
影响因子：
7.4
作者：
Ruban AV
通讯作者：
Ruban AV

A siphonous morphology affects light-harvesting modulation in the intertidal green macroalga Bryopsis corticulans (Ulvophyceae).

DOI：
10.1007/s00425-018-2854-5
发表时间：
2018-06
期刊：
Planta
影响因子：
4.3
作者：
Giovagnetti V;Han G;Ware MA;Ungerer P;Qin X;Wang WD;Kuang T;Shen JR;Ruban AV
通讯作者：
Ruban AV

Photosynthesis in Action

光合作用的作用