ENGINEERING SYNTHETIC MICROBIAL COMMUNITIES FOR BIOTECHNOLOGY
生物技术工程合成微生物群落
基本信息
- 批准号:BB/R01602X/1
- 负责人:
- 金额:$ 49.63万
- 依托单位:
- 依托单位国家:英国
- 项目类别:Research Grant
- 财政年份:2018
- 资助国家:英国
- 起止时间:2018 至 无数据
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
In nature, microorganisms live in communities -microbiota- where each member interacts with the others and with the environment. These microbial consortia are responsible for human, animal and crop health, ecological stability, and industrial bioprocesses. For example, in the recent years, human gut microbiota has been associated with many important diseases from cardiovascular diseases to diabetes. As another example, the most stable and robust industrial bioprocesses are carried out by microbial communities, such as the production of most fermented food and drink. Despite the very well known importance of microbiota, and our recent progress towards the identification of the members of the communities we still know very little about how these communities are established and maintained, which limit our possibilities to engineer them in order to tackle microbiota diseases or create new robust bioprocesses. To this end, there is significant interest in developing simplified microbial communities which can serve as a platform to address basic biological questions on microbial interactions and to create more efficient bioprocesses than those based on a single engineered microorganism. The work proposed here aims to design and build synthetic microbial communities using synthetic biology. Synthetic biology uses engineering principles in biological systems in a predictable, controllable and standardized manner in order to get new biological insights and create cells with improved abilities. This project, firstly, will generate stable synthetic microbial communities, and secondly, will use some of these communities to perform specific tasks, such as the production of biofuels and pharmaceuticals. This work will, therefore, be able to produce specialist strains valuable for use in biotechnology.To achieve these goals, I plan to use knowledge and tools gained from my previous experiences in microbial metabolite exchange, which is the basis for establishing communities. Engineering metabolism has proven useful to generate synthetic communities, as shown by my preliminary results and previous works, indicating the feasibility of the proposed work. Therefore, we will create a series of engineered microbial strains that are able to interact with each other in different manners and generate stable communities. In addition, the generated synthetic microbial communities will be used to create more complex population behaviours and learn by building how natural communities interact and evolve. This way, we will be able to control the abundance of the members in the population, we will generate new population-level functionalities, and we will re-create ecological relationships found in nature. This will generate important new insights for understanding how cells form and maintain microbiota.Finally, we will prove that synthetic microbial communities can be designed and build to improve the production of biotechnologically relevant compounds. Firstly, a community where two microorganisms cooperate to maximize the production of an industrial pharmaceutical will be engineered. Secondly, a community designed to select the best mutants to produce biodiesel will be generated and used. Both strategies are strongly supported by competitive industrial partners in the field, which indicates the enormous potential of this new technology. This project is, therefore, developing a new synthetic biology technology that can be used in a variety of fields, from helping us to better understand natural microbiota and tackle the associated diseases to create more robust and versatile bioprocesses, which may allow us to move towards the bioeconomy.
在自然界中,微生物生活在群落中-微生物群-每个成员与其他成员和环境相互作用。这些微生物菌群负责人类、动物和作物的健康、生态稳定性和工业生物过程。例如,近年来,人类肠道微生物群与从心血管疾病到糖尿病的许多重要疾病有关。作为另一个例子,最稳定和强大的工业生物过程是由微生物群落进行的,例如大多数发酵食品和饮料的生产。尽管微生物群的重要性众所周知,并且我们最近在识别社区成员方面取得了进展,但我们仍然对这些社区如何建立和维持知之甚少,这限制了我们对它们进行工程改造以解决微生物群疾病或创造新的强大生物过程的可能性。为此,人们对开发简化的微生物群落非常感兴趣,这些微生物群落可以作为一个平台来解决微生物相互作用的基本生物学问题,并创造比基于单一工程微生物的生物过程更有效的生物过程。本文提出的工作旨在使用合成生物学设计和构建合成微生物群落。合成生物学以可预测、可控制和标准化的方式在生物系统中使用工程原理,以获得新的生物学见解并创造具有改进能力的细胞。该项目首先将产生稳定的合成微生物群落,其次将利用其中一些群落执行特定任务,例如生产生物燃料和药品。因此,这项工作将能够产生在生物技术中有价值的专业菌株。为了实现这些目标,我计划使用从我以前的微生物代谢物交换经验中获得的知识和工具,这是建立社区的基础。工程代谢已被证明是有用的,以产生合成社区,如我的初步结果和以前的工作,表明拟议的工作的可行性。因此,我们将创造一系列工程微生物菌株,这些菌株能够以不同的方式相互作用,并产生稳定的群落。此外,生成的合成微生物群落将用于创造更复杂的种群行为,并通过建立自然群落如何相互作用和进化来学习。通过这种方式,我们将能够控制种群中成员的丰度,我们将产生新的种群功能,我们将重新创建自然界中的生态关系。这将为理解细胞如何形成和维持微生物群产生重要的新见解。最后,我们将证明合成微生物群落可以设计和构建,以提高生物技术相关化合物的生产。首先,将设计一个两种微生物合作以最大限度地生产工业药物的社区。其次,将产生和使用一个旨在选择最佳突变体以生产生物柴油的社区。这两项战略都得到了该领域有竞争力的工业合作伙伴的大力支持,这表明了这项新技术的巨大潜力。因此,该项目正在开发一种新的合成生物学技术,可用于各种领域,从帮助我们更好地了解自然微生物群和解决相关疾病,到创建更强大和多功能的生物过程,这可能使我们能够走向生物经济。
项目成果
期刊论文数量(10)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Vibrio natriegens genome-scale modeling reveals insights into halophilic adaptations and resource allocation.
Vibrio Natriegens基因组规模建模揭示了对卤素适应和资源分配的见解。
- DOI:10.15252/msb.202110523
- 发表时间:2023-04-12
- 期刊:
- 影响因子:9.9
- 作者:
- 通讯作者:
Spontaneously established syntrophic yeast communities improve bioproduction.
- DOI:10.1038/s41589-023-01341-2
- 发表时间:2023-08
- 期刊:
- 影响因子:14.8
- 作者:Aulakh, Simran Kaur;Vidal, Lara Selles;South, Eric J.;Peng, Huadong;Varma, Sreejith Jayasree;Herrera-Dominguez, Lucia;Ralser, Markus;Ledesma-Amaro, Rodrigo
- 通讯作者:Ledesma-Amaro, Rodrigo
Predicting inflation component drivers in Nigeria: a stacked ensemble approach.
预测尼日利亚的通货膨胀因素驱动因素:堆叠集成方法。
- DOI:10.1007/978-3-319-40715-9_9
- 发表时间:2023
- 期刊:
- 影响因子:0
- 作者:Akande EO
- 通讯作者:Akande EO
Resource-aware whole-cell model of division of labour in a microbial consortium for complex-substrate degradation.
- DOI:10.1186/s12934-022-01842-0
- 发表时间:2022-06-14
- 期刊:
- 影响因子:6.4
- 作者:
- 通讯作者:
Engineering Yarrowia lipolytica to produce nutritional fatty acids: Current status and future perspectives.
- DOI:10.1016/j.synbio.2022.06.002
- 发表时间:2022-12
- 期刊:
- 影响因子:4.8
- 作者:Cao, Lizhen;Yin, Mingxue;Shi, Tian-Qiong;Lin, Lu;Ledesma-Amaro, Rodrigo;Ji, Xiao-Jun
- 通讯作者:Ji, Xiao-Jun
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Rodrigo Ledesma Amaro其他文献
Rodrigo Ledesma Amaro的其他文献
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{{ truncateString('Rodrigo Ledesma Amaro', 18)}}的其他基金
Engineering Biology Hub for Microbial Food
微生物食品工程生物学中心
- 批准号:
BB/Y008510/1 - 财政年份:2024
- 资助金额:
$ 49.63万 - 项目类别:
Research Grant
A bench-top, parallel 8 bioreactor platform for optimizing the sustainable production of pharmaceuticals, biologics, materials, fuels and foods
用于优化药品、生物制剂、材料、燃料和食品可持续生产的台式、并行 8 生物反应器平台
- 批准号:
BB/X01911X/1 - 财政年份:2023
- 资助金额:
$ 49.63万 - 项目类别:
Research Grant
Yeast-based solutions for sustainable Aviation Fuels (YAF)
基于酵母的可持续航空燃料解决方案 (YAF)
- 批准号:
EP/Y031881/1 - 财政年份:2023
- 资助金额:
$ 49.63万 - 项目类别:
Research Grant
UNraveling, Identifying and COntrolling isogenic Heterogeneity
揭示、识别和控制同基因异质性
- 批准号:
EP/X026000/1 - 财政年份:2023
- 资助金额:
$ 49.63万 - 项目类别:
Fellowship
Harnessing Yarrowia metabolism to generate high value terpenoids
利用耶氏酵母代谢产生高价值萜类化合物
- 批准号:
BB/T013176/1 - 财政年份:2021
- 资助金额:
$ 49.63万 - 项目类别:
Research Grant
19-ERACoBioTech- 33 SyCoLim
19-ERACoBioTech-33 SyCoLim
- 批准号:
BB/T011408/1 - 财政年份:2020
- 资助金额:
$ 49.63万 - 项目类别:
Research Grant
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