EFFECTS OF PESTICIDE METHOXYCHLOR ON REPRODUCTION

农药甲氧基氯对繁殖的影响

• 批准号: 3252916
• 负责人: VICTOR P EROSCHENKO
• 金额: $ 7.31万
• 依托单位: UNIVERSITY OF IDAHO
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-26 至 1992-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3252916
• 关键词: electron microscopy; estrogens; estrus; female reproductive system; female reproductive system disorder; follicle stimulating hormone; genital secretion; histopathology; hormone regulation /control mechanism; laboratory mouse; luteinizing hormone; mature animal; neurohormones; newborn animals; occupational hazard; pesticide biological effect; pesticide interaction; reproduction; tissue /cell culture

The pesticide methoxychlor is presently in wide use as a substitute for the banned DDT. Biochemical research has established that the pesticide has estrogenic action; however, very little data are available concerning the effects of methoxychlor on mammalian reproductive organs. Reproductive systems of immature mice as well as other mammals appears highly sensitive to estrogenic action. This study proposes to measure the estrogenic and/or toxic effects of methoxychlor on the anatomy, physiology and cell biology of the reproductive system in neonatal and adult mice. To accomplish this goal, neonatal mice of Swiss-Webster strain will be exposed to methoxychlor and the following information collected: a) morphology of oviduct, uterus, cervix, vagina and ovaries, b) regularity of estrous cycles and onset of reproductive senescence, c) type of vaginal cornification, d) ovarian function and LH and FSH levels in ovariectomized mice, and e) patterns of secretions of proteinaceous molecules by endometrial cells in adults. The results obtained from this proposal will be valuable in establishing the mechanism of methoxychlor action in the reproductive organs of exposed newborn and adult mammals. In addition, the new data will be very useful in determining the impact of methoxychlor's wide use in the environment on the reproductive capabilities of non-target species and humans who, as a result of occupational exposure, may come in daily contact with this chemical.

农药甲氧氯目前被广泛用作 代替违禁DDT。 生化研究已有 确定农药具有雌激素作用。然而， 关于效果的数据很少 甲氧氯在哺乳动物生殖器官上。 生殖 未成熟小鼠以及其他哺乳动物的系统出现 对雌激素作用高度敏感。 这项研究提出了 测量甲氧氯的雌激素和/或毒性作用 生殖的解剖学，生理和细胞生物学 新生儿和成年小鼠的系统。 为了实现这一目标， 瑞士 - 温布斯特菌株的新生小鼠将暴露于 甲氧氯和以下信息收集：a） 卵形，子宫，子宫颈，阴道和卵巢的形态，b） 发情周期的规律性和生殖发作 衰老，c）阴道薄的类型，d）卵巢功能 卵巢切除小鼠的LH和FSH水平，以及e）模式 子宫内膜细胞中蛋白质分子的分泌物 成年人。 从本提案中获得的结果将是有价值的 在建立甲氧基氯在 裸露的新生儿和成人哺乳动物的生殖器官。 在 此外，新数据将非常有用 甲氧氯在环境中广泛使用的影响 非目标物种和人类的生殖能力， 由于职业曝光，可能每天接触 使用这种化学物质。

项目成果

Protein secretions in mouse uterus after methoxychlor or estradiol exposure.

甲氧滴滴涕或雌二醇暴露后小鼠子宫中的蛋白质分泌物。

• DOI: 10.1016/0890-6238(91)90007-3
• 发表时间: 1991
• 期刊: Reproductive toxicology (Elmsford, N.Y.)
• 作者: Rourke,AW;Eroschenko,VP;Washburn,LJ
• 通讯作者: Washburn,LJ

Ultrastructure of vagina and uterus in young mice after methoxychlor exposure.

甲氧滴滴涕暴露后幼鼠阴道和子宫的超微结构。

• DOI: 10.1016/0890-6238(91)90006-2
• 发表时间: 1991
• 期刊: Reproductive toxicology (Elmsford, N.Y.)
• 作者: Eroschenko,VP