The development of diagnostic techniques to assess anaemia in aquaculture reared Atlantic Salmon (Salmo Salar).

评估水产养殖大西洋鲑鱼（Salmo Salar）贫血的诊断技术的发展。

• 批准号: BB/S004467/1
• 负责人: Brian Quinn
• 金额: $ 22.73万
• 依托单位: University of the West of Scotland
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FS004467%2F1
• 关键词: development; diagnostic; techniques; assess; anaemia

Over the last 12 months anaemia has emerged as a significant threat to fish health in salmon aquaculture in Scotland. Anaemia occurs when there is a lack of red blood cells, reducing the oxygen carrying capacity of the blood and negatively impacting on fish health and production. Through recent SAIC project meetings with the aquaculture partners it was highlighted that the industry has limited practical methods to define the type of anaemia occurring, identify its source and subsequently develop a preventative strategy against it. The Scottish aquaculture industry is looking to establish a haematology monitoring programme, to characterise and quantify the impact of anaemia, but does not currently have access to automated technologies for fish haematology and is reliant on slow, labour intensive and subjective manual techniques. This lack of technological advancement has also resulted in a lack of reference data for the establishment of appropriate 'normal' background levels. The overall aim of this project is the development and validation of efficient methods to assess anaemia in Atlantic salmon in aquaculture that can be adopted by the industry and included into their regular fish health management programmes. This is being undertaken by the validation of existing veterinary and medical haematology devices for assessment of fish blood, which is considerably more difficult owing to fish blood cells being nucleated. However once the relevant techniques are established and validated they shall be used to investigate anaemia in fish and to put those results into the wider context of fish health measured using high throughput, automated medical technologies to assess clinical chemistry and immunology endpoints validated for use with fish in a separate (SAIC funded) project involving the same collaborative partners.The main benefit and impact of this research shall be improvements in fish health management, increasing fish welfare and growth, subsequently benefiting the industry by a reduction in costs, increased yield and improved product quality. The development of an appropriate diagnostic system to assess anaemia in salmon aquaculture requires; a) the development and validation of rapid, automated haematological methods for salmon blood, and b) the integration of this haematology data with relevant endpoints on fish health and disease. A holistic diagnostic approach is taken, placing the haematology findings in the context of fish health investigated by the high throughput assessment of biochemistry and immunology endpoints and histological analysis. This innovative approach provides information on the cause of anaemia, thereby allowing identification of solutions. Innovation is needed in the area of blood sample preparation and storage, data interpretation and particularly with the integration of physiological impacts associated with anaemia. There is a current lack of reference values in fish haematology and this work shall contribute towards the establishment of reference data of benefit to the salmon aquaculture industry in Scotland.In the final stage of the project anaemia in Atlantic salmon from various sites around Scotland shall be identified, defined, quantified and its impact measured using haematological, clinical chemistry and immunology high-throughput analysers integrated into a pro-active mechanised technologically advanced method to assess fish heath. These techniques are relevant to the salmon aquaculture industry in the UK and internationally and user friendly practical guides and protocols shall be developed along with an engagement workshop to offer hands on training and practice to encourage the inclusion of these techniques throughout the industry, greatly increasing the impact of this research.

在过去 12 个月中，贫血已成为苏格兰鲑鱼养殖业中鱼类健康的重大威胁。当红细胞缺乏时就会发生贫血，血液的携氧能力降低，并对鱼类的健康和生产产生负面影响。国家工商总局最近与水产养殖合作伙伴举行的项目会议强调，该行业在定义贫血类型、确定其来源以及随后制定预防策略方面的实用方法有限。苏格兰水产养殖业正在寻求建立血液学监测计划，以描述和量化贫血的影响，但目前无法获得鱼类血液学的自动化技术，并且依赖于缓慢、劳动密集型和主观的手工技术。技术进步的缺乏也导致缺乏建立适当的“正常”背景水平的参考数据。该项目的总体目标是开发和验证评估水产养殖中大西洋鲑鱼贫血的有效方法，该方法可供行业采用并纳入其常规鱼类健康管理计划。这是通过验证现有的用于评估鱼血的​​兽医和医学血液学设备来进行的，由于鱼血细胞有核，这要困难得多。然而，一旦建立并验证了相关技术，它们将用于调查鱼类贫血，并将这些结果纳入使用高通量、自动化医疗技术测量的更广泛的鱼类健康背景中，以评估在涉及相同合作伙伴的单独（国家工商总局资助）项目中验证用于鱼类的临床化学和免疫学终点。这项研究的主要好处和影响应是改善鱼类健康管理，提高鱼类福利和生长， 随后，该行业通过降低成本、提高产量和提高产品质量而受益。开发适当的诊断系统来评估鲑鱼水产养殖中的贫血症需要； a) 开发和验证鲑鱼血液的快速、自动化血液学方法，以及 b) 将血液学数据与鱼类健康和疾病的相关终点相整合。采用整体诊断方法，将血液学结果置于通过生物化学和免疫学终点以及组织学分析的高通量评估进行调查的鱼类健康背景中。这种创新方法提供了有关贫血原因的信息，从而可以确定解决方案。血样制备和储存、数据解释，特别是与贫血相关的生理影响的整合领域需要创新。目前鱼类血液学缺乏参考值，这项工作将有助于建立对苏格兰鲑鱼水产养殖业有益的参考数据。在项目的最后阶段，将使用血液学、临床化学和免疫学高通量分析仪将来自苏格兰各地的大西洋鲑鱼贫血症识别、定义、量化，并测量其影响，并将其纳入主动机械化技术先进的方法中。 评估鱼类健康状况。这些技术与英国和国际上的鲑鱼水产养殖业相关，应制定用户友好的实用指南和协议，并举办参与研讨会，提供实践培训和实践，鼓励将这些技术纳入整个行业，从而大大增加这项研究的影响。

项目成果

Anemia in salmon aquaculture: Scotland as a case study.

鲑鱼水产养殖中的贫血：苏格兰作为案例研究。

• DOI: 10.1016/j.aquaculture.2021.737313
• 发表时间: 2022-01-15
• 期刊: Aquaculture (Amsterdam, Netherlands)
• 作者: Currie AR;Cockerill D;Diez-Padrisa M;Haining H;Henriquez FL;Quinn B
• 通讯作者: Quinn B