Establishing the Efficacy, Safety and Persistence of biopesticides based on naturally occurring beneficial bacteria

确定基于天然有益细菌的生物农药的功效、安全性和持久性

• 批准号: BB/S007652/1
• 负责人: Eshwar Mahenthiralingam
• 金额: $ 63.56万
• 依托单位: CARDIFF UNIVERSITY
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FS007652%2F1
• 关键词: Establishing; Efficacy; Safety; Persistence; biopesticides

HARNESSING NATURAL BACTERIA TO PROTECT CROPS AND SUSTAIN AGRICULTUREThe aim of our research is to establish the fundamental scientific knowledge required to use naturally occuring plant protective bacteria in an effective, safe and environmentally non-persistent manner. Current agriculture is reliant on the use of chemical pesticides to protect crops from attack by disease-causing microorganisms. One such disease, known as damping off, is a major problem killing crop plants as they germinate. To feed the growing world population we will need to intensify agriculture and adding more chemical pesticides to protect crops. We cannot sustain high usage of pesticides, especially when they damage the environment and human health. Alternative approaches which are sustainable must be developed and natural bacterial biopesticides offer an attractive solution to enhance future agriculture and protect crops from disease.BURKHOLDERIA AMBIFARIA - A NATURALLY PLANT PROTECTIVE BACTERIASeveral natural bacteria live very closely with plants and provide protection against disease causing microbes. One group of bacteria, Burkholderia ambifaria (B. ambifaria), when applied as a seed coat are very good at protecting germinating crops from lethal attack by damping off fungi. This led to their safe use as biopesticides up to 1999 in the US, but during the 1990s, a related group of Burkholderia bacteria were found to cause lung infections in people with cystic fibrosis. At the time, the safety of B. ambifaria could not be fully defined, it was decided that registration of new biopesticides would not be permitted until they were demonstrated to have no health risks. Although the registered Burkholderia biopesticide products could still be used, the agricultural industry moved away from using them and expanded their use of chemical pesticides to sustain agriculture.UNDERSTANDING HOW BURKHOLDERIA AMBIFARIA PROTECTS PLANTS AND ENSURING IT IS SAFE AND ENVIRONMENTALLY NON-PERSISTENTUsing modern research techniques, we found B. ambifaria's plant protective ability was due to a set of genes that the bacterium uses to make an antifungal molecule called cepacin. When the cepacin-making genes were removed, plant protection was lost from the bacterial seed coat. By sequencing all the genes in B. ambifaria bacteria we also identified multiple new genes that make natural products potentially exploitable as biopesticide molecules, antibiotics or fine chemicals. These new findings have allowed us to map out a research program to re-purpose B. ambifaria as a biopesticide which will be carried out as the following four research objectives: 1. We will map all the molecules and pathways B. ambifaria uses to protect plants from attack by pathogens, specifically identifying those which are switched on when the bacteria grow on the exudates released by germinating crop seeds.2. We will engineer the cepacin pathway allowing it to be moved into other biotechnological bacteria and also exploring how it is able to make the unusual chemical structure of this biopesticidal molecule.3. We will improve the safety of B. ambifaria by identifying the genes it uses to cause disease and exploring different strategies to remove these genes. Infectious live bacteria can be used as vaccines when specific genes are removed, and the same approach will be exploited to make a safe biopesticide.4. We will explore what happens to B. ambifaria when it is placed in the soil as a seed coat and how it affects other soil microorganisms, so that we understand it's environmental persistence.Overall this integrated research programme will seek to deliver an effective, safe and environmentally non-persistent B. ambifaria biopesticide, which can significantly enhance and sustain agricultural production without the need to use chemical pesticides.

利用天然细菌来保护作物和可持续农业我们研究的目的是建立以有效，安全和环境非持久性的方式使用天然植物保护细菌所需的基本科学知识。目前的农业依赖于使用化学农药来保护作物免受致病微生物的侵袭。其中一种疾病，称为立枯病，是一个主要问题，杀死作物植物，因为他们发芽。为了养活不断增长的世界人口，我们将需要加强农业和增加更多的化学农药来保护作物。我们不能维持大量使用杀虫剂，特别是当它们损害环境和人类健康时。必须开发可持续的替代方法，天然细菌生物农药提供了一个有吸引力的解决方案，以提高未来的农业和保护作物免受疾病的侵害。BURKHOLDERIA AMBIFARIA -一种天然植物抗病性芽孢杆菌几种天然细菌与植物生活非常密切，并提供保护，以防止致病微生物。一组细菌，Burkholderia ambifaria（B. ambifaria），当作为种皮施用时，通过抑制真菌，非常好地保护发芽的作物免受致命攻击。这导致它们在1999年之前在美国安全地用作生物农药，但在20世纪90年代，发现一组相关的伯克霍尔德菌属细菌会导致囊性纤维化患者的肺部感染。当时，B的安全。由于不能完全界定生物农药的混淆性，因此决定在证明新的生物农药没有健康风险之前，不允许其注册。虽然已注册的伯克霍尔德氏菌生物农药产品仍然可以使用，但农业行业不再使用这些产品，而是扩大了化学农药的使用，以维持农业。了解伯克霍尔德氏菌如何对植物进行杀虫，并确保其安全性和环境非持久性使用现代研究技术，我们发现B。ambifaria的植物保护能力是由于一组基因，该细菌用来制造一种名为cepacin的抗真菌分子。当制造cepacin的基因被移除时，植物保护就从细菌种皮中消失了。通过对B中的所有基因进行测序。我们还鉴定了多个新的基因，这些基因使天然产物有可能用作生物农药分子、抗生素或精细化学品。这些新的发现使我们能够制定一个研究计划来重新利用B。本研究将作为生物农药ambifaria开展以下四个方面的研究目标：1.我们将绘制所有的分子和路径B。ambifaria用于保护植物免受病原体的攻击，特别是识别那些当细菌在发芽的作物种子释放的分泌物上生长时被打开的细菌。我们将设计头孢菌素途径，使其能够被转移到其他生物技术细菌中，并探索它如何能够使这种生物杀虫剂分子的不寻常的化学结构。我们将提高B的安全性。通过识别它用来引起疾病的基因，并探索不同的策略来去除这些基因，来研究ambifaria。当特定基因被去除时，传染性活细菌可以用作疫苗，并且同样的方法将被利用来制造安全的生物农药。我们将探讨B会发生什么。当它作为种皮被放置在土壤中时，它如何影响其他土壤微生物，以便我们了解它的环境持久性。总体而言，这项综合研究计划将寻求提供一种有效，安全和环境非持久性的B。ambifaria生物农药，可显著提高和维持农业生产，而无需使用化学农药。

项目成果

Exploration of polyyne biosynthetic gene cluster diversity in bacteria leads to the discovery of the Pseudomonas polyyne protegencin

对细菌中多炔生物合成基因簇多样性的探索导致了假单胞菌多炔保护蛋白的发现

• DOI: 10.1101/2021.03.05.433886
• 发表时间: 2021
• 作者: Mullins A

Natural bacterial biopesticides: weighing up the risk of pathogenic versus beneficial properties

天然细菌生物农药：权衡致病性与有益特性的风险

• 发表时间: 2019
• 期刊: Nature Microbiology Communities
• 作者: Mahenthiralingam E.

Genomic Assemblies of Members of Burkholderia and Related Genera as a Resource for Natural Product Discovery.

• DOI: 10.1128/mra.00485-20
• 发表时间: 2020-10-15
• 期刊: Microbiology resource announcements
• 影响因子: 0.8
• 作者: Mullins AJ;Jones C;Bull MJ;Webster G;Parkhill J;Connor TR;Murray JAH;Challis GL;Mahenthiralingam E
• 通讯作者: Mahenthiralingam E

Burkholderia bacteria: natural alternatives to synthetic pesticides

伯克霍尔德杆菌：合成农药的天然替代品

• 发表时间: 2019
• 期刊: Microbiology Today
• 作者: Mullins A.J.

Kill and cure: genomic phylogeny and bioactivity of Burkholderia gladioli bacteria capable of pathogenic and beneficial lifestyles.

• DOI: 10.1099/mgen.0.000515
• 发表时间: 2021-01
• 期刊: Microbial genomics
• 影响因子: 3.9
• 作者: Jones C;Webster G;Mullins AJ;Jenner M;Bull MJ;Dashti Y;Spilker T;Parkhill J;Connor TR;LiPuma JJ;Challis GL;Mahenthiralingam E
• 通讯作者: Mahenthiralingam E