RETINAL NEUROTRANSMITTERS AND SYNAPTIC CIRCUITRY

猫视网膜中特定化学成分的神经元亚群将是 通过使用神经递质物质作为细胞化学进行鉴定 光学和电子显微镜研究中的标记。与众不同 各亚群的形态特征和突触关系 将会被描述。放射自显影技术将用于研究 积累甘氨酸、GABA和牛磺酸的亚群。视网膜 以P物质等神经活性多肽为特征的神经元， 生长抑素和脑啡肽将通过两者的使用来识别 放射自显影和免疫细胞化学技术。电子密度 标记物5，6-二羟色胺将用于识别 使用多巴胺和吲哚胺作为传递体。功能通路将 通过放射自显影分析神经递质和 2-受控刺激条件下的脱氧葡萄糖摄取。的影响 光与暗、棒与锥的刺激，以及不同波长的光 将会被调查。化学成分之间的解剖关系 将通过使用双重标记来探索已定义的亚群 放射自显影将与高尔基体受孕相结合的研究， 免疫细胞化学和逆行转运。身份的鉴定 在形态上不同的视网膜神经元亚群利用 特定的化学物质和刺激条件的定义 这些影响这些发射器释放的因素将为 整合解剖学、神经化学和生理学数据。 猫视网膜Will结构和化学回路的阐明 提供有关视觉的初始处理的基础信息 哺乳动物视网膜中的信息。

Chemically specific subpopulations of neurons in the cat retinal will be identified through the use of neurotransmitter substances as cytochemical markers in light and electron microscopic studies. Distinctive morphological features and synaptic relationships of each subpopulation will be described. Autoradiographic techniques will be used to study subpopulations which accumulate glycine, GABA, and taurine. Retinal neurons characterized by neuroactive peptides including substance P, somatostatin, and enkephalin will be identified by the use of both autoradiographic and immunocytochemical techniques. The electron dense marker, 5,6-dihydroxytryptamine, will be used to identify neurons which employ dopamine and indoleamine as transmitters. Functional pathways will be studied through autoradiographic analysis of neurotransmitter and 2-deoxyglucose uptake under controlled stimulus conditions. The effects of light vs. dark, rod vs. cone stimulation, and varying wavelengths of light will be investigated. The anatomical relationships between chemically defined subpopulations will be explored through the use of double labeling studies in which autoradiography will be combined with Golgi impregnation, immunocytochemistry and retrograde transport. The identification of morphologically distinct subpopulations of retinal neurons which utilize specific chemical transmitters and the definition of stimulus conditions which influence the release of these transmitters will provide a basis for integration of anatomical, neurochemical, and physiological data. Elucidation of structural and chemical circuitry in the cat retina will furnish basis information regarding the initial processing of visual information in a mammalian retina.