RETINAL NEUROTRANSMITTERS AND SYNAPTIC CIRCUITRY

这项研究的目的是确定神经化学物质 猫视网膜的电路有助于其功能在处理 视觉信息。 具体而言，它旨在确定 形态学上确定的神经元的神经递质含量 亚群;阐明突触之间的关系 化学特异性细胞;测定递质的变化 在各种刺激条件下的水平;并确定 神经化学输入来定义神经节细胞的群体。 的 调查结果将与正在进行的研究相关， 这些神经元的形态学、生理学和药理学。 一 了解化学特异性神经元如何在神经元内相互作用。 视网膜将不仅有助于模型的发展， 视网膜功能，但也将有助于了解 神经系统的传递功能。 结合高尔基体和放射自显影技术将被采用 来观察积累甘氨酸、GABA和牛磺酸的细胞。 使用该技术进行定量将用于研究 特定神经元对假定递质的亲和力 不同的生理条件。 将产生抗甘氨酸、GABA、牛磺酸、 谷氨酸和天冬氨酸，用于免疫细胞化学 问题研究 每种抗血清将针对一种氨基酸 其已经与牛血清白蛋白结合。 Fab 将制备抗血清的片段并用于电子 显微镜 高尔基体和免疫细胞化学联合研究 将用于识别化学特异性细胞并确定 与其他神经元的关系。 双标签研究将 用于定义发射机共定位， 阐明化学特异性细胞之间的突触关系。

The goal of this study is to determine how the neurochemical circuitry of cat retina contributes to its function in processing visual information. Specifically, it seeks to determine the neurotransmitter content of morphologically defined neuronal subpopulations; to elucidate the synaptic relationships between chemically specific cells; to determine changes in transmitters levels under various stimulus conditions; and to ascertainthe neurochemical input to define populations of ganglion cell. The findings will be correlated with on-going studies of the morphology, physiology, and pharmacology of these neurons. A knowledge of how chemically specific neurons interact within the retina will contribute not only to the development of models of retinal function but will also assist in the understanding of transmitter function throughotu the nervous system. Combined Golgi and autoradiographic techniques will be employed to visualize cells which accumulate glycine, GABA, and taurine. Quantitation with this technique will be used to investigate the affinity of specific neurons for a putative transmitter under varying physiological conditions. Antisera will be generated against glycine, GABA, taurine, glutamate, and aspartate and used in immunocytochemical studies. Each antiserum will be directed against an amino acid which ahs been conjugated to bovine serum albumin. Fab fragments of the antisera will be prepared and used for electron microscopy. Combined Golgi and immunocytochemical studies will be used to identify chemically specific cells and to determine their relationships with other neurons. Double label studies will be employed to define transmitter co-localizations and to elucidate synaptic relationships between chemically specific cells.