GLYCEROLIPIDS/PROSTAGLANDINS BIOSYNTHESIS--OCULAR TISSUE

甘油脂/前列腺素生物合成——眼组织

• 批准号: 3258783
• 负责人: ATA A ABDEL-LATIF
• 金额: $ 12.36万
• 依托单位: AUGUSTA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-12-01 至 1992-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3258783
• 关键词: adrenergic agents; adrenergic receptor; antiinflammatory agents; arachidonate; chromatography; eicosanoid metabolism; enzyme inhibitors; extraocular muscle; eye agent; eye disorder; eye injury; fatty acid biosynthesis; genetic manipulation; inflammation; intraocular pressure; iris; irritation /irritant; laboratory rabbit; lipase; lipid biosynthesis; molecular pathology; radionuclide imaging /scanning; radiotracer; trabecular meshwork; trauma; uvea ciliary body

The overall objective of the Proposed research program is to throw more light on the molecular mechanisms underlying prostaglandin (PG) release, and to elucidate the relationship between adrenergic receptor activation and PG formation in ocular tissues. To achieve these objectives we propose to carry out the following experiments: (1) Investigate the release of basal (endogenous) arachidonic acid from phospholipids of rabbit and bovine irides and its subsequent conversion into PGE2. Methodology for these studies will be developed. (2) Investigate the effects of adrenergic drugs, Ca++, Ca++-inonophore and phospholipase A2 inhibitors on the basal release of arachidonate and PGE2 by rabbit and bovine irides. (3) Investigate the effects of in vivo electrical stimulation of the sympathetic nerve on the release of arachidonate and PGE2 by the rabbit iris. (4) Determine if any of the agents which were found to affect arachidonate and PGE2 release from ocular tissues can be used as anti-inflammatory drugs in the eye.

拟议研究计划的总体目标是投入更多 阐明前列腺素（PG）释放的分子机制， 并阐明肾上腺素能受体激活之间的关系 以及眼组织中 PG 的形成。 为了实现这些目标，我们建议 进行以下实验： (1) 调查释放 来自兔和牛磷脂的基础（内源）花生四烯酸 虹膜及其随后转化为 PGE2。 这些的方法论 将开展研究。 (2) 研究肾上腺素能的作用 基础药物、Ca++、Ca++-inonophore 和磷脂酶 A2 抑制剂 兔和牛虹膜释放花生四烯酸和 PGE2。 (3) 研究体内电刺激的影响 交感神经对兔释放花生四烯酸和 PGE2 的影响 虹膜。 (4) 确定是否存在任何被发现影响的因素 眼组织释放的花生四烯酸和 PGE2 可用作 眼睛里有抗炎药。