THE EFFECT OF OXIDATION UPON LENS TRANSPORT SYSTEMS

氧化对镜片传输系统的影响

• 批准号: 3259550
• 负责人: ABRAHAM SPECTOR
• 金额: $ 24.96万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-12-01 至 1991-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3259550
• 关键词: NAD(H) phosphate; adenosine diphosphate; adenosine triphosphate; aminoacid transport; biological transport; cataract; drug screening /evaluation; eye pharmacology; glutathione; hydrogen peroxide; lactates; lens; membrane permeability; nicotinamide adenine dinucleotide; oxidation; oxidation reduction reaction; photochemistry; potassium; radioimmunoassay; scintillation counter; sodium

The aims of this project are to delineate the mechanism by which oxidation causes changes in lens transport systems. Three model systems will be used to examine transport. H2O2, hyperbaric O2 and photo-oxidation. Na/K ATPase, Ca ATPase and amino acid transport will be investigated. The impact of oxidative insult to the pump systems upon important metabolic parameters such as ATP, NAD(P) and NAD(P)H, lactate and glutathione will be examined. The model systems will be compared to human cataract to ascertain similarities in the pattern of cataract development. The origin of aqueous H2O2 and its relationship to ascorbate will also be investigated as well as initiation of a drug screening program to protect the lens membrane from oxidative insult.

这个项目的目的是描绘氧化的机制， 导致透镜运输系统的变化。 将使用三个模型系统 检查运输。 H2 O2、高压O2和光氧化。 Na/K ATP酶、Ca-ATP酶和氨基酸转运。 对泵系统的氧化损伤对重要代谢的影响 参数如ATP、NAD（P）和NAD（P）H、乳酸盐和谷胱甘肽将被 考察 该模型系统将与人类白内障进行比较以确定 白内障发展模式的相似性。 H2 O2水溶液的来源及其与抗坏血酸盐的关系也将被讨论。 调查以及启动药物筛选计划，以保护 使透镜膜免受氧化损伤。