COOPERATIVE CATARACT RESEARCH GROUP CONSORTIUM

这项研究的目的是描绘涉及到的机制 老年性白内障的形成。方法是研究 动物晶状体的生化和形态变化及其相互关系 人类老年性白内障的观察与变化少校 实验室的假设是，氧化侮辱可能是一种引发 或导致白内障形成的早期事件。答辩审查 针对氧化侮辱的系统正在进行中。研究正在进行中 膜Na/K-ATPase、Ca-ATPase等转运系统的研究进展 结构，高分子量共价连接的蛋白质聚集体； H_2O_2、光化学和高压氧对运输、代谢和 晶状体的蛋白质和脂肪组分；细胞骨架结构的变化 随着年龄的增长和白内障的形成，氧化对分子的影响 组织生物学和γ-晶体蛋白的表达；多胺 相互作用、磷酸化和钙离子对分子结构的影响。 氧化损伤诱导蛋白质变性的生物物理研究 目前也在接受调查。在所有情况下都对人类晶状体进行了研究 按CCRG分类的正常和白内障正在进行中。对.的使用 分析数据的先知系统是我们程序中不可或缺的一部分。 这个应用程序是专门支持、收集和分类的 人力材料，并支付数据分析所涉及的费用 先知系统。

The aim of the research is to delineate the mechanisms involved in the formation of the senile type of cataract. The approach is to study biochemical and morphological changes in animal lenses and correlate such observations with changes observed in human senile cataract. The major hypothesis of the laboratory is that oxidative insult may be an initiating or early event contributing to cataract formation. Examination of defense systems against oxidative insult are being conducted. Studies are in progress upon Na/K ATPase, Ca ATPase and other transport systems; membrane structure, high molecular weight covalently linked protein aggregates; H2O2, photochemical and hyperbaric O2 effects on transport, metabolism and protein and lipid fractions of the lens; changes in cytoskeleton structure with aging and cataract formation, effects of oxidation on the molecular biology of the tissue and expression of gamma crystallin; polyamine interactions, phosphorylation and Ca++ effects on molecular structure. Bio-physical studies of protein denaturation induced by oxidative insult are also under investigation. In all cases study of human lenses both normal and cataract, classified by CCRG procedures are ongoing. The use of the PROPHET system to analyze data is an integral part of our procedures. This application is specifically to support, collection and classification of human material and to cover expenses involved with analysis of data by the PROPHET system.