COOPERATIVE CATARACT RESEARCH GROUP CONSORTIUM

白内障研究合作联盟

• 批准号: 3259681
• 负责人: ABRAHAM SPECTOR
• 金额: $ 2.02万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-03-01 至 1989-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3259681
• 关键词: aging; biomedical facility; cataract; cooperative study; disease /disorder classification; eye bank /preservation; human tissue; information systems; oxidation

The aim of the research is to delineate the mechanisms involved in the formation of the senile type of cataract. The approach is to study biochemical and morphological changes in animal lenses and correlate such observations with changes observed in human senile cataract. The major hypothesis of the laboratory is that oxidative insult may be an initiating or early event contributing to cataract formation. Examination of defense systems against oxidative insult are being conducted. Studies are in progress upon Na/K ATPase, Ca ATPase and other transport systems; membrane structure, high molecular weight covalently linked protein aggregates; H2O2, photochemical and hyperbaric O2 effects on transport, metabolism and protein and lipid fractions of the lens; changes in cytoskeleton structure with aging and cataract formation, effects of oxidation on the molecular biology of the tissue and expression of gamma crystallin; polyamine interactions, phosphorylation and Ca++ effects on molecular structure. Bio-physical studies of protein denaturation induced by oxidative insult are also under investigation. In all cases study of human lenses both normal and cataract, classified by CCRG procedures are ongoing. The use of the PROPHET system to analyze data is an integral part of our procedures. This application is specifically to support, collection and classification of human material and to cover expenses involved with analysis of data by the PROPHET system.

这项研究的目的是描述参与的机制， 老年性白内障的形成。 方法是研究 动物晶状体的生物化学和形态学变化， 在人类老年性白内障中观察到的变化。 主要 实验室的假设是氧化损伤可能是 或导致白内障形成的早期事件。 答辩审查 正在实施抗氧化损伤的系统。 研究正在 Na/K ATP酶、Ca ATP酶等转运系统的研究进展 结构，高分子量共价连接的蛋白质聚集体; H2O2、光化学和高压O2对运输、代谢和 透镜的蛋白质和脂质部分;细胞骨架结构的变化 随着衰老和白内障的形成，氧化对分子的影响 组织生物学和γ晶体蛋白的表达;多胺 相互作用、磷酸化和Ca ++对分子结构的影响。 氧化损伤引起蛋白质变性的生物物理学研究 也在接受调查。 在所有人类晶状体的研究中， 根据CCRG程序分类的正常和白内障正在进行中。 使用 PROPHET系统分析数据是我们程序的一个组成部分。 这个应用程序是专门支持，收集和分类 人力资源，并支付与数据分析有关的费用， 先知系统