OUTER PLEXIFORM LAYER STRUCTURE AND FUNCTION

建议继续研究视网膜的结构和功能 拉纳的神经元。水平细胞和受体端突的研究 将在最初的赠款期间完成。这份续签建议书 涉及双极细胞和无长突细胞。 程序包括细胞内记录以建立基本的接受性 光场特性、HRP注入和形态计量学研究 注射细胞的电子显微镜。这些数据将与 高尔基体制剂和系列光、电子的定量数据 标准制剂的显微镜检查。 对于两极细胞，这项研究将把细胞功能与树突细小联系起来。 结构，识别的受体接触，一般形态，水平 分枝与触点的精细结构和分布 塔伦多里亚。对于无长突细胞，细胞功能将与 工艺模式和程度，触点的精细结构和分布。 含有儿茶酚胺和吲哚胺的视网膜内细胞 以甲醛诱导的光镜和电子显微镜为特征 组织荧光和选择性退变程序。它的形态特征 这些细胞将与高尔基体和HRP的结果相关联 学习。

It is proposed to continue a study of the structure and function of retinal neurones of Rana. Studies of horizontal cells and receptor telodendria will be completed during the initial grant period. This renewal proposal concerns bipolar and amacrine cells. Procedures include intracellular recording to establish basic receptive field properties and HRP injectioned and morphometric studies by light and electron microscopy of injected cells. These data will be correlated with quantitative data from Golgi preparations and serial light and electron microscopy of standard preparations. For bipolars, the study will correlate cell function with dendritic fine structure, identified receptor contacts, general morphology, level of branching and the fine structure and distribution of contacts on telodendria. For amacrine cells, cell function will be correlated with process pattern and extent, fine structure and distribution of contacts. Catecholamine and Indoleamine containing cells of the inner retina will be characterized by light and electron microscopy using formaldehyde induced histofluorescence and selective degeneration procedures. The morphology of these cells will be correlated with the results of the Golgi and HRP studies.