THE PATHOGENESIS OF NERVE FIBER IN GLAUCOMA

该建议涉及青光眼神经纤维损伤的发病机制。 大量的实验和临床研究表明， 这种疾病中的纤维损伤是视神经盘的巩膜板。 两种流行的理论认为，由于神经纤维的压迫， 椎间盘结构变形或神经纤维缺血， 椎板灌注不足。 两种理论都是基于 在椎间盘和神经纤维层中， 识别样式disc. 过去对这种视网膜病变的研究产生了相互矛盾的结果。 我们建议检查视网膜视神经纤维的组织， 视网膜、椎间盘、神经、视交叉和神经束。 单根纤维 将通过轴突内注射HRP进行染色，并以步进方式追踪 连续切片。 我们将研究青光眼的灵长类动物模型，以确定青光眼的大小，起源和 单个受损纤维的路径。 这些研究还将提供一个 眼巩膜板早期变化的形态计量学描述 高血压 一项辅助研究将利用灵长类青光眼模型来确定 局灶性模式ERG和模式VER的有用性，以揭示早期 视神经纤维功能障碍

This proposal concerns the pathogenesis of nerve fiber damage in glaucoma. A number of experimental and clinical studies suggest the site of nerve fiber injury in this disease is the lamina scleralis of the optic disc. Two popular theories suggest as causative compression of nerve fibers due to distortion of disc structure or ischemia of nerve fibers due to defective perfusion of the lamina. Both theories are based on the presence of a high order of retinotopy in the disc and in the nerve fiber layer near the disc. Past studies of this retinotopy produced conflicting results. We propose to examine the retinopic organization of optic nerve fibers in retina, disc, nerve, chiasm and tract to the monkey. Individual fibers will be stained by intra-axonal injection of HRP and traced in stepped serial sections. We will study a primate model of glaucoma to determine the size, origin and pathway of individual injured fibers. These studies will also provide a morphometric description of early changes in the lamina scleralis in ocular hypertension. An ancillary study will make use of the primate glaucoma model to determine the usefulness of the focal pattern ERG and pattern VER to reveal early optic nerve fiber dysfunction.