RHODOPSIN STRUCTURE AND ENVIRONMENT IN VISION

视觉中的视紫红质结构和环境

• 批准号: 3257654
• 负责人: ARLENE Diane ALBERT
• 金额: $ 14.39万
• 依托单位: STATE UNIVERSITY OF NEW YORK AT BUFFALO
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-05-01 至 1987-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3257654
• 关键词: X ray crystallography; acidity /alkalinity; adenosinetriphosphatase; calcium; cholesterol; chromatography; conformation; dialysis; electron microscopy; eye regeneration; lipid structure; membrane lipids; nuclear magnetic resonance spectroscopy; phospholipids; retina degeneration; retinitis pigmentosa; rhodopsin; rod cell; ultracentrifugation; vision

The rod cells of vertebrate retinas are responsible for vision at low levels of light. The visual pigment, rhodopsin, absorbs light as the primary event. This is followed by a series of events which leads to hyperpolarization of the plasma membrane of the rod outer segment and results in a nerve impulse. This proposal deals with the molecular mechanisms involving rhodopsin function and the phospholipid matrix which are important in early events of visual transduction. The stability of the disk lipid bilayer will be investigated after specific modifications of the lipid matrix both in the presence and absence of rhodopsin. These modifications include changes in lipid composition, Ca2+ concentrations, and pH. The effect these modifications exert on rhodopsin will also be investigated with respect to the protein stability and function. The rhodopsin molecule will be subjected to modifications at specific sites. The effect of these modifications will be investigated with respect to rhodopsin function and membrane stability to identify more specifically how rhodopsin functions in visual transduction. The stabilization of the disk bilayers with respect to the role of both protein (rhodopsin and opsin) and phospholipid composition should be of value in understanding membrane degenerative diseases such as retinitis pigmentosa. The role of himicholinium-containing phospholipids in experimentally induced retinal degeneration will be directly addressed. The role of cholesterol in modulating rhodopsin function will be determined.

脊椎动物视网膜的杆细胞负责低处的视力 光的水平。 视觉色素，视紫红质，吸收光作为 主要事件。 接下来是一系列事件，导致 杆外段的质膜超极化和 导致神经冲动。 该建议涉及分子 涉及视紫红质功能和磷脂基质的机制 在视觉转导的早期事件中很重要。 磁盘脂质双层的稳定性将在特定之后进行研究 在存在和不存在的情况下，脂质基质的修饰 视紫红质。 这些修饰包括脂质组成的变化，Ca2+ 浓度和pH。 这些修饰对视紫红质的影响 还将研究有关蛋白质稳定性和 功能。 Rhodopsin分子将在特定上进行修饰 站点。 这些修改的效果将被研究 视紫红质的功能和膜稳定性，以更具体地识别 Rhodopsin如何在视觉转导中起作用。 磁盘双层相对于两者的作用的稳定 蛋白质（Rhodopsin和Opsin）和磷脂组成应为 理解膜退行性疾病（例如视网膜炎）的价值 色素 在实验中含有himicholin的磷脂的作用 诱导的视网膜变性将直接解决。 将确定胆固醇在调节视紫红蛋白功能中的作用。