NEURAL AND PHARMACOLOGICAL CONTROLS OF AQUEOUS DYNAMICS

这项建议的目标是研究神经和药理学。 房水动力学的控制。神经机制将调查 神经肽(胰多肽、血管活性物质)的眼部效应 肠多肽等。)和房水的生理调节剂 动力学。这些药物将通过外周(局部的， 房内、玻璃体内)和中央(第三脑室和下丘脑 微量注射)给药途径。神经通路将是 通过消融颈上神经节来观察， 岩浅神经和三叉神经。药理控制 将特别关注眼部碳酸酐酶的研究 活性抑制剂；降眼压作用 局部应用多巴胺及特异性多巴胺受体(DA1)的作用 和DA2)激动剂和拮抗剂；以及 外用大麻素化合物。房水动力学将包括 眼内压，流出设施，巩膜上静脉压，房水 体液流量，以及眼部血流量和血流量。这些研究涉及到 眼内压的调节。结果将提供信息 神经肽作为房水生理调节剂的作用 动力学，并可能提供关于潜在的病理生理学的线索 青光眼。眼压的药物调节将提供 对正常房水生理和可能的新青光眼的洞察 治疗剂。

The objectives of this proposal are to study neural and pharmacological controls of aqueous humor dynamics. Neural mechanisms will investigate the ocular effects of neuropeptides (pancreatic polypeptide, vasoactive intestinal polypeptide, etc.) and physiological regulators of aqueous humor dynamics. These agents will be studied via peripheral (topical, intracameral, intravitreal) and central (third ventricle and hypothalamic microinjection) routes of administration. Neural pathways are to be investigated by ablation of the superior cervical ganglion, greater superficial petrosal nerve and trigeminal nerve. Pharmacological controls will study ocular carbonic anhydrase with special attention to topically active inhibitory agents; the intraocular pressure lowering effect of topical dopamine as well as the effect of specific dopamine receptor (DA1 and DA2) agonists and antagonists; and the intraocular pressure effect of topical cannabinoid compounds. Aqueous humor dynamics will include intraocular pressure, outflow facility, episcleral venous pressure, aqueous humor flow, and ocular blood flow and volume. These studies relate to the regulation of intraocular pressure. The results will provide information on the role of neuropeptides as physiological regulators of aqueous humor dynamics and may provide clues about the underlying pathophysiology of glaucoma. Pharmacological modulation of intraocular pressure will provide insight into normal aqueous humor physiology and possibly new glaucoma therapeutic agents.