ELECTROPHYSIOLOGY OF CILIARY BODY TRANSPORT PROCESSES

这项建议的长期目标是研究细胞 纤毛产生房水的机制 上皮组织。具体目标是体外电生理学研究。 睫状体上皮分离制剂的转运过程。 这些实验将提供有关正常生理的信息 活动性血管紧张素转换酶的机制及药物诱导的改变 产生房水的体液。减少房水形成的方法 内科治疗是眼球突出症的常见治疗方法。 与青光眼相关的压力。关于细胞机制的知识 负责房水制作的人将提供对 房水形成的正常率和可能的药物作用 房水产生率降低的机制。 独立的睫状体制剂被安装用于测量 跨上皮电参数(电势差、短路 海流)、净流体输送和单向通量。这些研究 表示组织中活跃的运输和液体流动的巩固 保持正常解剖关系的准备工作 睫状突的双层上皮层。常规微管 用于测量膜电位的穿透研究是在 结合跨上皮测量。这些研究提供了 单个睫状体上皮细胞与离子的关系 移动和运输系统。此外，细胞内离子导入 荧光染料荧光黄的注射用于研究GAP 为细胞间通讯提供途径的连接(细胞- 细胞耦合)，从而允许细胞作为合胞体发挥作用。 电生理检查被用来研究以下类别 药物：(一)卤化麻醉剂，(二)肾上腺素能激动剂和 神经肽Y与肾上腺素能激动剂的相互作用(III) 巴比妥酸盐；和(Iv)叠氮钠。这些实验将提供 关于活性生产水溶液的机理的信息 幽默，并可能导致新的和独特的抗青光眼药物设计 抑制房水产生的不同顺序步骤。

Long-term objective of this proposal is the investigation of cellular mechanisms relating to the production of aqueous humor by the ciliary epithelium. Specific aims are the in vitro electrophysiologic study of transport processes on isolated preparations of the ciliary epithelium. These experiments will furnish information on normal physiological mechanisms and pharmacological-induced alterations involved in the active production of aqueous humor. Reduction of aqueous humor formation by medical therapy is a frequent treatment of the elevated intraocular pressure associated with glaucoma. Knowledge regarding cellular mechanisms responsible for aqueous humor production will provide insights into the normal rate of aqueous humor formation and possible pharmacologic mechanisms for the reduction in the rate of aqueous humor production. Isolated ciliary body preparations are mounted for measurement of transepithelial electrical parameters (potential difference, short-circuit current), net fluid transport, and unidirectional fluxes. These studies represent a consolidation of active transport and fluid flow in a tissue preparation maintaining the normal anatomic relationships between the double epithelial layers of the ciliary processes. Conventional micropipet impalement studies for measurement of membrane potential are performed in conjunction with the transepithelial measurements. These studies provide information on individual ciliary epithelial cells in relationship to ion movements and transport systems. In addition, intracellular iontophoretic injection of the fluorescent dye Lucifer Yellow is performed to study gap junctions which provide a pathway for intercellular communication (cell- cell coupling), thereby allowing cells to function as a syncytium. Electrophysiologic investigations are used to study the following classes of drugs: (i) halogenated anesthetic agents, (ii) adrenergic agonists and the interaction of neuropeptide Y with the adrenergic agonists, (iii) barbiturates, and (iv) sodium azide. These experiments will furnish information on mechanisms involved in the active production of aqueous humor and may result in new and unique antiglaucoma agents designed to inhibit the different sequential steps in aqueous humor production.