ROLE OF NEAR-UV LIGHT IN RETINAL DEGENERATION AND AGING

近紫外光在视网膜退化和老化中的作用

• 批准号: 3258976
• 负责人: LAURENCE M RAPP
• 金额: $ 19.21万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-03-01 至 1995-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3258976
• 关键词: aging; all trans retinol; animal age group; antioxidants; autoradiography; catalase; disease /disorder prevention /control; electron microscopy; electroretinography; environmental toxicology; glutathione peroxidase; laboratory rabbit; laboratory rat; light adverse effect; lipid peroxides; melanins; nonvisual photosensitivity; radiobiology; retina degeneration; rod cell; spectrometry; superoxide dismutase; tissue /cell culture; ultraviolet radiation; visual photoreceptor; visual pigments

There is increasing concern that exposure to ultraviolet light, found in solar radiation, may be involved in the etiology of ocular disorders such as age-related macular degeneration. There is, however, an incomplete understanding of the mechanisms by which light stress leads to retinal pathology. The proposed research will investigate the role of near- ultraviolet light in causing retinal degeneration and aging. Recent findings in our laboratory have established pigmented rats as a suitable experimental animal for studying near-ultraviolet light toxicity of the retina. Using primarily the rat model and in some cases rabbits, experiments are specifically designed to 2) characterize near-ultraviolet light retinal damage in sub-primate mammals and distinguish it from the well-known form of retinal damage in these species that is caused by green light (i.e. rhodopsin-mediated damage); 2) evaluate the role of various endogenous chromophores in mediating or influencing near-ultraviolet retinal damage; 3) examine the possible involvement of lipid peroxidation in the biochemical etiology of near-UV retinal toxicity; 4) examine the influence of near-ultraviolet light stress on retinal aging; and 5) evaluate the relative contribution of different visible wavelength bands in causing retinal lesions produced by operating microscope light. A quantitative, multidisciplinary approach will be applied to these studies using electroretinographic, morphometric and various biochemical techniques. The intention of the proposed studies is to provide an in-depth analysis of the mechanisms by which near-ultraviolet light leads to retinal pathology. The information obtained from these studies could help to predict the conditions predisposing the retinal to near-ultraviolet damage and to devise strategies for preventing and managing photically-induced retinal disease.

人们越来越担心，暴露于紫外线，发现在 太阳辐射，可能与眼部疾病的病因有关， 老年性黄斑变性 然而，有一个不完整的 了解光应激导致视网膜病变的机制 病理 这项研究将调查近- 紫外线会导致视网膜退化和老化。 最近 我们实验室的研究结果已经确定有色大鼠是合适的 近紫外光毒性实验动物 视网膜。 主要使用大鼠模型，在某些情况下使用兔子， 实验是专门设计来2）表征近紫外 亚灵长类哺乳动物的视网膜轻度损伤， 一种众所周知的视网膜损伤形式是由绿色引起的 光（即视紫红质介导的损伤）; 2）评估各种因素的作用 内源性发色团介导或影响近紫外 视网膜损伤; 3）检查脂质过氧化的可能参与 近紫外线视网膜毒性的生化病因学; 4）检查 近紫外光应激对视网膜老化的影响;以及5） 评估不同可见光波段的相对贡献 导致手术显微镜光产生的视网膜损伤。 一 这些研究将采用定量、多学科的方法 使用视网膜电图、形态测量和各种生物化学 技术. 拟议研究的目的是深入分析 近紫外线导致视网膜病变的机制。 从这些研究中获得的信息可以帮助预测 条件诱发视网膜近紫外线损伤， 制定预防和管理光诱导视网膜病变的策略， 疾病