ROLE OF NEAR-UV LIGHT IN RETINAL DEGENERATION AND AGING

近紫外光在视网膜退化和老化中的作用

• 批准号: 3258981
• 负责人: LAURENCE M RAPP
• 金额: $ 19.11万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-03-01 至 1995-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3258981
• 关键词: aging; all trans retinol; animal age group; antioxidants; autoradiography; catalase; disease /disorder prevention /control; electron microscopy; electroretinography; environmental toxicology; glutathione peroxidase; laboratory rabbit; laboratory rat; light adverse effect; lipid peroxides; melanins; nonvisual photosensitivity; radiobiology; retina degeneration; rod cell; spectrometry; superoxide dismutase; tissue /cell culture; ultraviolet radiation; visual photoreceptor; visual pigments

There is increasing concern that exposure to ultraviolet light, found in solar radiation, may be involved in the etiology of ocular disorders such as age-related macular degeneration. There is, however, an incomplete understanding of the mechanisms by which light stress leads to retinal pathology. The proposed research will investigate the role of near- ultraviolet light in causing retinal degeneration and aging. Recent findings in our laboratory have established pigmented rats as a suitable experimental animal for studying near-ultraviolet light toxicity of the retina. Using primarily the rat model and in some cases rabbits, experiments are specifically designed to 2) characterize near-ultraviolet light retinal damage in sub-primate mammals and distinguish it from the well-known form of retinal damage in these species that is caused by green light (i.e. rhodopsin-mediated damage); 2) evaluate the role of various endogenous chromophores in mediating or influencing near-ultraviolet retinal damage; 3) examine the possible involvement of lipid peroxidation in the biochemical etiology of near-UV retinal toxicity; 4) examine the influence of near-ultraviolet light stress on retinal aging; and 5) evaluate the relative contribution of different visible wavelength bands in causing retinal lesions produced by operating microscope light. A quantitative, multidisciplinary approach will be applied to these studies using electroretinographic, morphometric and various biochemical techniques. The intention of the proposed studies is to provide an in-depth analysis of the mechanisms by which near-ultraviolet light leads to retinal pathology. The information obtained from these studies could help to predict the conditions predisposing the retinal to near-ultraviolet damage and to devise strategies for preventing and managing photically-induced retinal disease.

人们越来越担心暴露在紫外线下，发现在