PROBLEMS IN EYE INFECTIONS

我们建议研究局部眼因素在发病机制中的作用。 和控制眼睛的单纯疱疹病毒(HSV)感染。在 这里提出的实验我们将：(1)测试角膜标本 疱疹病毒性眼病患者与对照病例潜伏期比较 角膜病毒感染；(2)建立角膜动物模型 潜伏期；(3)检查局部(局部)介体的影响，特别是 肾上腺素能药物对实验性复发性眼部病毒脱落的影响 动物模型；(4)评价相同介质对单纯疱疹病毒感染的影响 角膜器官培养和细胞培养系统中的复制。 将确定角膜潜伏单纯疱疹病毒的患病率和发病机制 通过共培养和免疫化学染色从 角膜移植术中的疱疹眼病患者。我们还将发展 单纯疱疹病毒在动物角膜中潜伏期的体外模型及比较 在受感染的角膜和神经节中。 我们还将研究潜伏感染患者的眼部病毒脱落情况。 局部应用肾上腺素能药物和其他介体的动物 (例如前列腺素、干扰素)来检验局部 具体影响眼组织支持能力的因素 单纯疱疹病毒复制。这些化合物将在潜伏感染的人体内进行测试 动物、角膜器官培养和细胞培养系统。这样做的目的是 研究是检查使用特定介体的局部治疗方法 有可能预防或增强人类复发的疱疹眼病。

We propose to study the effect of local ocular factors on the pathogenesis and control of herpes simplex virus (HSV) infections of the eye. In the experiments proposed here we will: (1) Test corneal specimens from patients with herpetic eye disease and from control cases to reveal latent virus infection of the cornea; (2) develop animal models of corneal latency; (3) examine the effect of topical (local) mediators, particularly adrenergic drugs, on recurrent viral shedding from the eye in experimental animal models; (4) evaluate the effect of these same mediators on HSV replication in corneal organ culture and cell culture systems. To prevalence and pathogenesis of latent HSV in cornea will be determined by co-cultivation and immunochemical staining of corneas removed from patients with herpetic eye disease at keratoplasty. We will also develop an in vitro model of HSV latency in animal corneas and compare reactivation in infected corneas and ganglia. We will also study the ocular shedding of virus in latently infected animals following locally applied adrenergic drugs and other mediators (e.g. prostaglandins, interferons) to test the hypothesis that local factors specifically affect the ability of the ocular tissues to support HSV replication. These compounds will be tested in latently infected animals, corneal organ cultures and cell culture systems. The goal of this research is to examine local treatment with specific mediators for their potential to prevent or enhance recurrent herpetic eye disease in humans.